Restriction map for
pF1KB9940
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI AspEI AspI AvaI
BalI BbeI BbrPI BcgI BcnI
BfrI BglI BglII BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FauI FseI HaeII HincII
HindII HindIII HpaI KasI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1157 GGTACC G^GTACC
AcyI 1918 GGCGTC G[GA]^CG[CT]C
AlwNI 1223 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 860 GGGCCC GGGCC^C
Asp700I 1189 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1157 GGTACC G^GTACC
AviII 929 TGCGCA TGC^GCA
AvrII 1317 CCTAGG C^CTAGG
BamHI 285 GGATCC G^GATCC
BanI 1157 GGTACC G^G[CT][GA]CC
BbvCI 1538 CCTCAGC CCTCAGC(-5/-2)
BciVI 181 GGATAC GTATCC(6/5)
BlnI 1317 CCTAGG C^CTAGG
BpmI 61 CTGGAG CTGGAG(16/14)
BsaAI 964 CACGTA [CT]AC^GT[GA]
BsaBI 1176 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1918 GGCGTC G[GA]^CG[CT]C
BsaI 803 GGTCTC GGTCTC(1/5)
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BsmAI 804 GTCTC GTCTC(1/5)
BsmFI 648 GGGAC GGGAC(10/14)
BspHI 1058 TCATGA T^CATGA
BspLU11I 1148 ACATGT A^CATGT
BsrFI 1084 ACCGGC [GA]^CCGG[CT]
BstXI 963 CCACGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 970 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 1084 ACCGGC [GA]^CCGG[CT]
DraI 1485 TTTAAA TTT^AAA
DrdI 589 GACAATAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 88 CCATGG C^C[GA][CT]GG
EcoNI 1623 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1843 GAATTC G^AATTC
EcoT22I 1496 ATGCAT ATGCA^T
FspI 929 TGCGCA TGC^GCA
HgaI 1919 GCGTC GACGC(5/10)
Hin1I 1918 GGCGTC G[GA]^CG[CT]C
KpnI 1157 GGTACC GGTAC^C
MamI 1176 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 88 CCATGG C^CATGG
NsiI 1496 ATGCAT ATGCA^T
PshAI 170 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1058 TCATGA T^CATGA
SacI 36 GAGCTC GAGCT^C
SapI 99 GCTCTTC GCTCTTC(1/4)
ScaI 714 AGTACT AGT^ACT
SwaI 1484 ATTTAAAT ATTT^AAAT
Tsp45I 587 GTGAC ^GT[GC]AC
XcmI 677 CCATGATTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1189 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 30 CGCG CG^CG
1367 CGCG
AflIII 936 ACGTGT A^C[GA][CT]GT
1148 ACATGT
Alw44I 500 GTGCAC G^TGCAC
1421 GTGCAC
ApaLI 500 GTGCAC G^TGCAC
1421 GTGCAC
AvaII 66 GGACC G^G[AT]CC
252 GGACC
BclI 572 TGATCA T^GATCA
1715 TGATCA
BseRI 21 CTCCTC GAGGAG(10/8)
865 CTCCTC
BsmI 1494 GAATGC GAATGC(1/-1)
1572 GAATGC
BsrDI 1750 CATTGC GCAATG(2/0)
1894 GCAATG
BsrI 1052 ACTGG ACTGG(1/-1)
487 CCAGT
BssKI 297 CCTGG ^CC[ACGT]GG
1834 CCTGG
BstNI 297 CCTGG CC^[AT]GG
1834 CCTGG
BstUI 30 CGCG CG^CG
1367 CGCG
CfoI 31 GCGC GCG^C
930 GCGC
DraII 566 AGGCCCT [GA]G^G[ACGT]CC[CT]
860 GGGCCCT
DraIII 332 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1424 CACGATGTG
EarI 100 CTCTTC CTCTTC(1/4)
109 CTCTTC
EcoO109I 566 AGGCCCT [GA]G^G[ACGT]CC[CT]
860 GGGCCCT
EcoRII 297 CCTGG ^CC[AT]GG
1834 CCTGG
FbaI 572 TGATCA T^GATCA
1715 TGATCA
HapII 312 CCGG C^CGG
1085 CCGG
HhaI 31 GCGC GCG^C
930 GCGC
HinP1I 31 GCGC G^CGC
930 GCGC
HinfI 735 GATTC G^A[ACGT]TC
1927 GATTC
HpaII 312 CCGG C^CGG
1085 CCGG
HphI 690 GGTGA GGTGA(8/7)
575 TCACC
Ksp632I 100 CTCTTC CTCTTC(1/4)
109 CTCTTC
MaeII 936 ACGT A^CGT
965 ACGT
MspA1I 58 CCGCTG C[AC]G^C[GT]G
262 CAGCGG
MspI 312 CCGG C^CGG
1085 CCGG
MvaI 297 CCTGG CC^[AT]GG
1834 CCTGG
MvnI 30 CGCG CG^CG
1367 CGCG
NspI 1148 ACATGT [GA]CATG^[CT]
1201 GCATGT
PflMI 238 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCATGTGGTGG
ScrFI 297 CCTGG CC^[ACGT]GG
1834 CCTGG
SfaNI 1549 GATGC GCATC(5/9)
1288 GCATC
SpeI 171 ACTAGT A^CTAGT
666 ACTAGT
TfiI 735 GATTC G^A[AT]TC
1927 GATTC
Van91I 238 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCATGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BstAPI
BstF5I BstYI Cfr13I DdeI DpnII
EcoT14I Fnu4HI FokI HaeIII ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I SfcI StyI Tru9I TseI
Tsp509I TspRI XhoII