Restriction map for
pF1KB9859
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I EarI Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 59 GGCGCC G[GA]^CG[CT]C
AflIII 743 ACATGT A^C[GA][CT]GT
AlwNI 895 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 134 GGGCCC GGGCC^C
AspHI 195 GAGCTC G[AT]GC[AT]^C
BbeI 59 GGCGCC GGCGC^C
BbsI 708 GAAGAC GAAGAC(2/6)
BciVI 1047 GTATCC GTATCC(6/5)
BpuAI 708 GAAGAC GAAGAC(2/6)
BsaHI 59 GGCGCC G[GA]^CG[CT]C
BseRI 286 CTCCTC GAGGAG(10/8)
BsiHKAI 195 GAGCTC G[AT]GC[AT]^C
BspLU11I 743 ACATGT A^CATGT
BsrBI 262 CCGCTC CCGCTC(-3/-3)
BssHII 293 GCGCGC G^CGCGC
BstAPI 831 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 636 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 222 GACGCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 519 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 620 GGCCGGCC GGCCGG^CC
HgaI 222 GACGC GACGC(5/10)
Hin1I 59 GGCGCC G[GA]^CG[CT]C
HinfI 849 GAATC G^A[ACGT]TC
KasI 59 GGCGCC G^GCGCC
KspI 49 CCGCGG CCGC^GG
MaeIII 88 GTCAC ^GT[ACGT]AC
MslI 753 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 59 GGCGCC GG^CGCC
NspI 743 ACATGT [GA]CATG^[CT]
PaeR7I 664 CTCGAG C^TCGAG
PstI 829 CTGCAG CTGCA^G
PvuII 556 CAGCTG CAG^CTG
SacI 195 GAGCTC GAGCT^C
SacII 49 CCGCGG CCGC^GG
SexAI 899 ACCTGGT A^CC[AT]GGT
SfcI 829 CTGCAG C^T[GA][CT]AG
SfoI 59 GGCGCC GGC^GCC
SmaI 610 CCCGGG CCC^GGG
TfiI 849 GAATC G^A[AT]TC
Tsp45I 88 GTCAC ^GT[GC]AC
XhoI 664 CTCGAG C^TCGAG
XmaCI 610 CCCGGG C^CCGGG
XmaI 610 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 158 AGCGCT AGC^GCT
447 AGCGCT
BanII 134 GGGCCC G[GA]GC[CT]^C
195 GAGCTC
BcgI 341 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
773 GCAAGGAGATCG
BsgI 691 CTGCAC GTGCAG(16/14)
838 GTGCAG
BsiEI 309 CGGCCG CG[GA][CT]^CG
623 CGGCCG
BsrI 142 CCAGT ACTGG(1/-1)
641 CCAGT
DpnII 780 GATC ^GATC
822 GATC
EagI 309 CGGCCG C^GGCCG
623 CGGCCG
EclXI 309 CGGCCG C^GGCCG
623 CGGCCG
Eco47III 158 AGCGCT AGC^GCT
447 AGCGCT
Eco52I 309 CGGCCG C^GGCCG
623 CGGCCG
MboI 780 GATC ^GATC
822 GATC
NaeI 12 GCCGGC GCC^GGC
621 GCCGGC
NdeII 780 GATC ^GATC
822 GATC
NgoMIV 12 GCCGGC G^CCGGC
621 GCCGGC
NlaIII 705 CATG CATG^
744 CATG
PpuMI 202 GGGACCT [GA]G^G[AT]CC[CT]
242 GGGACCC
Sau3AI 780 GATC ^GATC
822 GATC
SmlI 664 CTCGAG C^T[CT][GA]AG
682 CTCAAG
TaqI 665 TCGA T^CGA
782 TCGA
TthHB8I 665 TCGA T^CGA
782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BbvCI BbvI BcnI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV RsaI
Sau96I ScrFI SfaNI TseI TspRI