Restriction map for
pF1KB9921
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DpnII DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 673 GTCTAC GT^[AC][GT]AC
AciI 832 GCGG CCGC(-3/-1)
AcsI 182 GAATTT [GA]^AATT[CT]
AcyI 670 GGCGTC G[GA]^CG[CT]C
ApoI 182 GAATTT [GA]^AATT[CT]
AspHI 558 GTGCTC G[AT]GC[AT]^C
AspI 16 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGAG C^[CT]CG[GA]G
BanII 249 GAGCCC G[GA]GC[CT]^C
BfaI 319 CTAG C^TAG
BpmI 458 CTGGAG CTGGAG(16/14)
BsaHI 670 GGCGTC G[GA]^CG[CT]C
BsaWI 410 ACCGGA [AT]^CCGG[AT]
BsiHKAI 558 GTGCTC G[AT]GC[AT]^C
BsiYI 520 CCATTATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 520 CCATTATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 14 GAGAC GTCTC(1/5)
BsoBI 9 CCCGAG C^[CT]CG[GA]G
Bsp1407I 471 TGTACA T^GTACA
BspHI 809 TCATGA T^CATGA
BsrDI 852 CATTGC GCAATG(2/0)
BsrGI 471 TGTACA T^GTACA
BstEII 354 GGTGACC G^GT[ACGT]ACC
BstPI 354 GGTGACC G^GT[ACGT]ACC
Cac8I 667 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 536 GCGC GCG^C
DraI 594 TTTAAA TTT^AAA
EaeI 833 CGGCCA [CT]^GGCC[GA]
EarI 908 CTCTTC CTCTTC(1/4)
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
EcoT14I 254 CCAAGG C^C[AT][AT]GG
HaeII 535 GGCGCT [GA]GCGC^[CT]
HapII 411 CCGG C^CGG
HgaI 671 GCGTC GACGC(5/10)
HhaI 536 GCGC GCG^C
Hin1I 670 GGCGTC G[GA]^CG[CT]C
HinP1I 536 GCGC G^CGC
HpaII 411 CCGG C^CGG
Ksp632I 908 CTCTTC CTCTTC(1/4)
MaeI 319 CTAG C^TAG
MnlI 771 CCTC CCTC(7/6)
MspA1I 830 CAGCGG C[AC]G^C[GT]G
MspI 411 CCGG C^CGG
NlaIV 248 GGAGCC GG[ACGT]^[ACGT]CC
PflFI 16 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 809 TCATGA T^CATGA
SexAI 350 ACCAGGT A^CC[AT]GGT
SspBI 471 TGTACA T^GTACA
StyI 254 CCAAGG C^C[AT][AT]GG
Tth111I 16 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BmyI 249 GAGCCC G[AGT]GC[ACT]^C
558 GTGCTC
Bsp1286I 249 GAGCCC G[AGT]GC[ACT]^C
558 GTGCTC
BsrI 199 ACTGG ACTGG(1/-1)
363 CCAGT
BssKI 351 CCAGG ^CC[ACGT]GG
900 CCTGG
BstNI 351 CCAGG CC^[AT]GG
900 CCTGG
EcoRII 351 CCAGG ^CC[AT]GG
900 CCTGG
HaeIII 769 GGCC GG^CC
834 GGCC
HinfI 215 GATTC G^A[ACGT]TC
372 GATTC
MboII 369 GAAGA GAAGA(8/7)
909 TCTTC
MslI 135 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
630 CATTCAAATG
MvaI 351 CCAGG CC^[AT]GG
900 CCTGG
MwoI 313 GCTTTCCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
826 GCAGCAGCGGC
NlaIII 309 CATG CATG^
810 CATG
PstI 71 CTGCAG CTGCA^G
824 CTGCAG
ScrFI 351 CCAGG CC^[ACGT]GG
900 CCTGG
SfaNI 337 GATGC GCATC(5/9)
223 GCATC
TfiI 215 GATTC G^A[AT]TC
372 GATTC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AfaI AluI BbvI BsaJI BstF5I
DdeI Fnu4HI FokI HphI ItaI
MaeIII MseI RsaI SfcI Tru9I
TseI Tsp45I Tsp509I TspRI