Restriction map for
pF1KB9918
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluNI MroI MscI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 631 GACGTC GACGT^C
AccI 3 GTCTAC GT^[AC][GT]AC
AccII 671 CGCG CG^CG
AcyI 631 GACGTC G[GA]^CG[CT]C
AocI 1319 CCTCAGG CC^T[ACGT]AGG
BanI 588 GGCACC G^G[CT][GA]CC
BanII 327 GGGCTC G[GA]GC[CT]^C
BciVI 897 GTATCC GTATCC(6/5)
BsaHI 631 GACGTC G[GA]^CG[CT]C
BsiEI 41 CGGCCG CG[GA][CT]^CG
BspLU11I 1172 ACATGT A^CATGT
BsrFI 43 GCCGGT [GA]^CCGG[CT]
BstUI 671 CGCG CG^CG
BstXI 1403 CCATCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1319 CCTCAGG CC^T[ACGT]AGG
Cfr10I 43 GCCGGT [GA]^CCGG[CT]
EaeI 41 CGGCCG [CT]^GGCC[GA]
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 1319 CCTCAGG CC^T[ACGT]AGG
EcoNI 1617 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 44 CCGG C^CGG
HgaI 672 GCGTC GACGC(5/10)
Hin1I 631 GACGTC G[GA]^CG[CT]C
HpaII 44 CCGG C^CGG
MluI 670 ACGCGT A^CGCGT
MspA1I 639 CAGCTG C[AC]G^C[GT]G
MspI 44 CCGG C^CGG
MvnI 671 CGCG CG^CG
NcoI 1357 CCATGG C^CATGG
PflMI 414 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 639 CAGCTG CAG^CTG
SexAI 1266 ACCTGGT A^CC[AT]GGT
SfaNI 861 GATGC GCATC(5/9)
SpeI 1566 ACTAGT A^CTAGT
SphI 437 GCATGC GCATG^C
SspI 262 AATATT AAT^ATT
Van91I 414 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1466 CCAAAGAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 515 AAATTT [GA]^AATT[CT]
759 AAATTT
AflIII 670 ACGCGT A^C[GA][CT]GT
1172 ACATGT
AlwNI 8 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1262 CAGAACCTG
Aor51HI 450 AGCGCT AGC^GCT
1037 AGCGCT
ApoI 515 AAATTT [GA]^AATT[CT]
759 AAATTT
BbsI 36 GTCTTC GAAGAC(2/6)
634 GTCTTC
BmyI 327 GGGCTC G[AGT]GC[ACT]^C
1455 GTGCCC
BpuAI 36 GTCTTC GAAGAC(2/6)
634 GTCTTC
Bsp1286I 327 GGGCTC G[AGT]GC[ACT]^C
1455 GTGCCC
BstYI 807 AGATCC [GA]^GATC[CT]
1231 AGATCC
DraII 71 AGGTCCT [GA]G^G[ACGT]CC[CT]
464 GGGACCT
DsaI 1113 CCGTGG C^C[GA][CT]GG
1357 CCATGG
Eco47III 450 AGCGCT AGC^GCT
1037 AGCGCT
EcoO109I 71 AGGTCCT [GA]G^G[ACGT]CC[CT]
464 GGGACCT
EcoT14I 414 CCAAGG C^C[AT][AT]GG
1357 CCATGG
HaeIII 42 GGCC GG^CC
700 GGCC
MflI 807 AGATCC [GA]^GATC[CT]
1231 AGATCC
MslI 996 CACTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1404 CATCCCAATG
NspI 437 GCATGC [GA]CATG^[CT]
1172 ACATGT
PleI 789 GACTC GAGTC(4/5)
852 GACTC
PpuMI 71 AGGTCCT [GA]G^G[AT]CC[CT]
464 GGGACCT
SfcI 5 CTACAG C^T[GA][CT]AG
1438 CTACAG
StyI 414 CCAAGG C^C[AT][AT]GG
1357 CCATGG
XhoII 807 AGATCC [GA]^GATC[CT]
1231 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmFI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeII HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I