Restriction map for
pF1KB6996
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 696 TCCGGA T^CCGGA
AflIII 173 ACATGT A^C[GA][CT]GT
AlwI 484 GGATC GGATC(4/5)
AviII 53 TGCGCA TGC^GCA
BbsI 334 GTCTTC GAAGAC(2/6)
BcnI 47 CCGGG CC^[GC]GG
BpmI 616 CTCCAG CTGGAG(16/14)
BpuAI 334 GTCTTC GAAGAC(2/6)
BsaWI 696 TCCGGA [AT]^CCGG[AT]
BseAI 696 TCCGGA T^CCGGA
BsgI 553 GTGCAG GTGCAG(16/14)
BsmAI 244 GAGAC GTCTC(1/5)
BspEI 696 TCCGGA T^CCGGA
BspLU11I 173 ACATGT A^CATGT
BspMI 144 ACCTGC ACCTGC(4/8)
BsrDI 511 GCAATG GCAATG(2/0)
BstYI 484 GGATCT [GA]^GATC[CT]
Cac8I 56 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 54 GCGC GCG^C
DdeI 458 CTGAG C^T[ACGT]AG
DpnII 485 GATC ^GATC
DraIII 160 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 254 CCATGG C^C[GA][CT]GG
EcoRI 668 GAATTC G^AATTC
EcoT14I 254 CCATGG C^C[AT][AT]GG
FauI 18 GCGGG CCCGC(4/6)
FspI 53 TGCGCA TGC^GCA
HhaI 54 GCGC GCG^C
HinP1I 54 GCGC G^CGC
MboI 485 GATC ^GATC
MflI 484 GGATCT [GA]^GATC[CT]
MroI 696 TCCGGA T^CCGGA
NciI 47 CCGGG CC^[GC]GG
NcoI 254 CCATGG C^CATGG
NdeI 70 CATATG CA^TATG
NdeII 485 GATC ^GATC
Sau3AI 485 GATC ^GATC
SmlI 241 CTTGAG C^T[CT][GA]AG
StyI 254 CCATGG C^C[AT][AT]GG
Tsp45I 361 GTCAC ^GT[GC]AC
XhoII 484 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 390 GAATTT [GA]^AATT[CT]
668 GAATTC
AlwNI 103 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
482 CAGGATCTG
ApoI 390 GAATTT [GA]^AATT[CT]
668 GAATTC
AvaII 251 GGACC G^G[AT]CC
343 GGACC
Bsp1407I 120 TGTACA T^GTACA
603 TGTACA
BsrGI 120 TGTACA T^GTACA
603 TGTACA
BstF5I 570 GGATG GGATG(2/0)
726 GGATG
Cfr13I 251 GGACC G^G[ACGT]CC
343 GGACC
EaeI 11 CGGCCA [CT]^GGCC[GA]
544 CGGCCA
FokI 570 GGATG GGATG(9/13)
726 GGATG
HaeIII 12 GGCC GG^CC
545 GGCC
HapII 47 CCGG C^CGG
697 CCGG
HinfI 81 GAATC G^A[ACGT]TC
733 GAATC
HpaII 47 CCGG C^CGG
697 CCGG
HphI 159 TCACC GGTGA(8/7)
757 TCACC
MaeIII 361 GTCAC ^GT[ACGT]AC
454 GTTAC
MspI 47 CCGG C^CGG
697 CCGG
NspI 173 ACATGT [GA]CATG^[CT]
677 ACATGC
Sau96I 251 GGACC G^G[ACGT]CC
343 GGACC
SfaNI 111 GCATC GCATC(5/9)
436 GCATC
SspBI 120 TGTACA T^GTACA
603 TGTACA
TfiI 81 GAATC G^A[AT]TC
733 GAATC
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
357 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsaJI BsrI BssKI BstNI EcoRII
Fnu4HI ItaI MaeI MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
PstI PvuII RsaI ScrFI SfcI
Tru9I TseI Tsp509I