Restriction map for
pF1KB6992
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 267 GGCGCC G[GA]^CG[CT]C
AocI 247 CCTAAGG CC^T[ACGT]AGG
ApaI 308 GGGCCC GGGCC^C
Asp700I 691 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 361 GAGCAC G[AT]GC[AT]^C
AvaI 380 CCCGGG C^[CT]CG[GA]G
BanI 267 GGCGCC G^G[CT][GA]CC
BbeI 267 GGCGCC GGCGC^C
BlpI 59 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 59 GCTCAGC GC^T[ACGT]AGC
BsaHI 267 GGCGCC G[GA]^CG[CT]C
BsiHKAI 361 GAGCAC G[AT]GC[AT]^C
BsmBI 47 CGTCTC CGTCTC(1/5)
BsoBI 380 CCCGGG C^[CT]CG[GA]G
Bsp1407I 413 TGTACA T^GTACA
BsrGI 413 TGTACA T^GTACA
BstF5I 708 GGATG GGATG(2/0)
BstNI 121 CCAGG CC^[AT]GG
Bsu36I 247 CCTAAGG CC^T[ACGT]AGG
CelII 59 GCTCAGC GC^T[ACGT]AGC
DpnII 643 GATC ^GATC
EarI 503 GAAGAG CTCTTC(1/4)
Eco81I 247 CCTAAGG CC^T[ACGT]AGG
EcoRII 121 CCAGG ^CC[AT]GG
FokI 708 GGATG GGATG(9/13)
Hin1I 267 GGCGCC G[GA]^CG[CT]C
KasI 267 GGCGCC G^GCGCC
Ksp632I 503 GAAGAG CTCTTC(1/4)
MaeII 46 ACGT A^CGT
MboI 643 GATC ^GATC
MseI 605 TTAA T^TAA
MvaI 121 CCAGG CC^[AT]GG
NarI 267 GGCGCC GG^CGCC
NdeII 643 GATC ^GATC
NlaIII 333 CATG CATG^
NspI 332 GCATGC [GA]CATG^[CT]
PleI 3 GACTC GAGTC(4/5)
Sau3AI 643 GATC ^GATC
SfaNI 66 GATGC GCATC(5/9)
SfoI 267 GGCGCC GGC^GCC
SmaI 380 CCCGGG CCC^GGG
SphI 332 GCATGC GCATG^C
SspBI 413 TGTACA T^GTACA
TaqI 645 TCGA T^CGA
TfiI 156 GATTC G^A[AT]TC
Tru9I 605 TTAA T^TAA
TthHB8I 645 TCGA T^CGA
XmaCI 380 CCCGGG C^CCGGG
XmaI 380 CCCGGG C^CCGGG
XmnI 691 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Aor51HI 175 AGCGCT AGC^GCT
480 AGCGCT
BanII 308 GGGCCC G[GA]GC[CT]^C
384 GGGCTC
BsiEI 135 CGGCCG CG[GA][CT]^CG
163 CGGCCG
BsmAI 280 GAGAC GTCTC(1/5)
48 GTCTC
BssHII 391 GCGCGC G^CGCGC
393 GCGCGC
EagI 135 CGGCCG C^GGCCG
163 CGGCCG
EclXI 135 CGGCCG C^GGCCG
163 CGGCCG
Eco47III 175 AGCGCT AGC^GCT
480 AGCGCT
Eco52I 135 CGGCCG C^GGCCG
163 CGGCCG
EcoT14I 192 CCAAGG C^C[AT][AT]GG
704 CCTTGG
HinfI 3 GACTC G^A[ACGT]TC
156 GATTC
HphI 640 GGTGA GGTGA(8/7)
295 TCACC
StyI 192 CCAAGG C^C[AT][AT]GG
704 CCTTGG
Tsp509I 607 AATT ^AATT
648 AATT
TspRI 417 CAGTG CA[GC]TG[ACGT][ACGT]^
744 CACTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstUI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI KspI
MboII MnlI MspA1I MspI MvnI
MwoI NciI NlaIV RsaI SacII
Sau96I ScrFI TseI