Restriction map for
pF1KB6991
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HpaI HphI
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I XbaI XcmI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 54 CGCG CG^CG
AclI 477 AACGTT AA^CGTT
AfaI 540 GTAC GT^AC
AlwI 226 GATCC GGATC(4/5)
Aor51HI 362 AGCGCT AGC^GCT
ApaI 180 GGGCCC GGGCC^C
AspI 23 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 138 CCCGGG C^[CT]CG[GA]G
BanI 483 GGTGCC G^G[CT][GA]CC
BanII 180 GGGCCC G[GA]GC[CT]^C
BbvCI 98 CCTCAGC CCTCAGC(-5/-2)
BfaI 122 CTAG C^TAG
BglI 647 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 409 CTGGAG CTGGAG(16/14)
BsaBI 246 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 21 GAGAC GTCTC(1/5)
BsmBI 21 GAGACG CGTCTC(1/5)
BsmFI 143 GTCCC GGGAC(10/14)
BsoBI 138 CCCGGG C^[CT]CG[GA]G
BspMI 639 ACCTGC ACCTGC(4/8)
BsrBI 192 GAGCGG CCGCTC(-3/-3)
BsrI 174 ACTGG ACTGG(1/-1)
BstAPI 634 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 54 CGCG CG^CG
DdeI 99 CTCAG C^T[ACGT]AG
DpnII 226 GATC ^GATC
DraIII 395 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 382 GAAGAG CTCTTC(1/4)
Eco47III 362 AGCGCT AGC^GCT
EcoT14I 575 CCAAGG C^C[AT][AT]GG
HaeII 362 AGCGCT [GA]GCGC^[CT]
HindIII 83 AAGCTT A^AGCTT
Ksp632I 382 GAAGAG CTCTTC(1/4)
MaeI 122 CTAG C^TAG
MaeII 478 ACGT A^CGT
MamI 246 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 226 GATC ^GATC
MvnI 54 CGCG CG^CG
NdeII 226 GATC ^GATC
NlaIII 433 CATG CATG^
PflFI 23 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 637 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 270 GAGTC GAGTC(4/5)
Psp1406I 477 AACGTT AA^CGTT
PstI 202 CTGCAG CTGCA^G
RsaI 540 GTAC GT^AC
Sau3AI 226 GATC ^GATC
SmaI 138 CCCGGG CCC^GGG
StyI 575 CCAAGG C^C[AT][AT]GG
TfiI 309 GATTC G^A[AT]TC
Tth111I 23 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 637 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 49 GTCTAC GT^[AC][GT]AC
272 GTCTAC
AlwNI 101 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
538 CAGTACCTG
BcnI 138 CCCGG CC^[GC]GG
139 CCGGG
BsgI 232 GTGCAG GTGCAG(16/14)
511 GTGCAG
BsiYI 420 CCTGCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
637 CCACCTGCTGG
BslI 420 CCTGCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
637 CCACCTGCTGG
BstF5I 71 CATCC GGATG(2/0)
252 CATCC
FauI 255 CCCGC CCCGC(4/6)
631 CCCGC
FokI 71 CATCC GGATG(9/13)
252 CATCC
HapII 43 CCGG C^CGG
139 CCGG
HgaI 23 GACGC GACGC(5/10)
505 GACGC
HinfI 270 GAGTC G^A[ACGT]TC
309 GATTC
HpaII 43 CCGG C^CGG
139 CCGG
MboII 60 GAAGA GAAGA(8/7)
382 GAAGA
MspI 43 CCGG C^CGG
139 CCGG
NciI 138 CCCGG CC^[GC]GG
139 CCGGG
PpuMI 117 GGGTCCT [GA]G^G[AT]CC[CT]
141 GGGTCCC
SfaNI 246 GATGC GCATC(5/9)
70 GCATC
SfcI 202 CTGCAG C^T[GA][CT]AG
274 CTACAG
TaqI 224 TCGA T^CGA
326 TCGA
TthHB8I 224 TCGA T^CGA
326 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeIII MnlI MspA1I MvaI MwoI
NlaIV PvuII Sau96I ScrFI TseI
Tsp45I TspRI