Restriction map for
pF1KB6989
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DpnII DraI DraII DraIII EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboI MboII MfeI MflI MluI
MroI MseI MunI MvnI NaeI
NarI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 643 AAATTC [GA]^AATT[CT]
AhdI 154 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 188 GTGCAC G^TGCAC
AlwNI 203 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 188 GTGCAC G^TGCAC
ApoI 643 AAATTC [GA]^AATT[CT]
AspEI 154 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 542 TGGCCA TGG^CCA
BanII 250 GAGCTC G[GA]GC[CT]^C
BseRI 505 GAGGAG GAGGAG(10/8)
BsmAI 498 GTCTC GTCTC(1/5)
BsmI 410 GAATGC GAATGC(1/-1)
BsrDI 629 GCAATG GCAATG(2/0)
BsrFI 601 GCCGGT [GA]^CCGG[CT]
BsrI 267 ACTGG ACTGG(1/-1)
BssSI 172 CACGAG CACGAG(-5/-1)
BstXI 62 CCACCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 601 GCCGGT [GA]^CCGG[CT]
DrdI 610 GACCATCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 154 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 255 CCATGG C^C[AT][AT]GG
HaeII 515 GGCGCT [GA]GCGC^[CT]
HgaI 529 GACGC GACGC(5/10)
MaeII 375 ACGT A^CGT
MaeIII 586 GTCAC ^GT[ACGT]AC
MluNI 542 TGGCCA TGG^CCA
MscI 542 TGGCCA TGG^CCA
NcoI 255 CCATGG C^CATGG
NspI 494 GCATGT [GA]CATG^[CT]
PflMI 216 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 122 CTGCAG CTGCA^G
SacI 250 GAGCTC GAGCT^C
SexAI 581 ACCTGGT A^CC[AT]GGT
SfcI 122 CTGCAG C^T[GA][CT]AG
SfiI 225 GGCCGACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 35 CCCGGG CCC^GGG
SmlI 561 CTCAAG C^T[CT][GA]AG
StyI 255 CCATGG C^C[AT][AT]GG
TfiI 109 GATTC G^A[AT]TC
Tsp45I 586 GTCAC ^GT[GC]AC
Tsp509I 644 AATT ^AATT
TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 216 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 65 CCATCATGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 35 CCCGGG C^CCGGG
XmaI 35 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BglI 226 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
551 GCCGGGATGGC
BpmI 247 CTGGAG CTGGAG(16/14)
268 CTGGAG
BstF5I 474 GGATG GGATG(2/0)
555 GGATG
CfoI 177 GCGC GCG^C
516 GCGC
DsaI 221 CCGTGG C^C[GA][CT]GG
255 CCATGG
FokI 474 GGATG GGATG(9/13)
555 GGATG
HhaI 177 GCGC GCG^C
516 GCGC
HinP1I 177 GCGC G^CGC
516 GCGC
MslI 63 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
69 CATGGCAGTG
PleI 417 GACTC GAGTC(4/5)
617 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstNI Cac8I Cfr13I
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PvuII Sau96I
ScrFI SfaNI TaqI TseI TthHB8I