Restriction map for
pF1KB7812
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HphI ItaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI TaqI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 320 GTAGAC GT^[AC][GT]AC
AciI 75 GCGG CCGC(-3/-1)
AflIII 1615 ACATGT A^C[GA][CT]GT
AlwI 806 GATCC GGATC(4/5)
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
Asp700I 513 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 248 CCCGAG C^[CT]CG[GA]G
BbsI 121 GTCTTC GAAGAC(2/6)
BclI 155 TGATCA T^GATCA
BpuAI 121 GTCTTC GAAGAC(2/6)
BsaI 251 GAGACC GGTCTC(1/5)
BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 1765 ACCGGA [AT]^CCGG[AT]
BsmFI 3 GGGAC GGGAC(10/14)
BsoBI 248 CCCGAG C^[CT]CG[GA]G
BspLU11I 1615 ACATGT A^CATGT
BsrDI 54 GCAATG GCAATG(2/0)
FbaI 155 TGATCA T^GATCA
HinfI 280 GATTC G^A[ACGT]TC
MspA1I 73 CAGCGG C[AC]G^C[GT]G
NlaIV 3 GGGACC GG[ACGT]^[ACGT]CC
NspI 1615 ACATGT [GA]CATG^[CT]
PshBI 339 ATTAAT AT^TAAT
SexAI 116 ACCTGGT A^CC[AT]GGT
SfaNI 375 GATGC GCATC(5/9)
SfcI 108 CTACAG C^T[GA][CT]AG
SspI 432 AATATT AAT^ATT
StuI 182 AGGCCT AGG^CCT
TfiI 280 GATTC G^A[AT]TC
XmnI 513 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 26 TGGCCA TGG^CCA
215 TGGCCA
BfaI 590 CTAG C^TAG
1409 CTAG
BsgI 68 CTGCAC GTGCAG(16/14)
1061 CTGCAC
BsiYI 255 CCTTTGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
629 CCTTTAACTGG
BslI 255 CCTTTGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
629 CCTTTAACTGG
BstF5I 21 GGATG GGATG(2/0)
363 GGATG
DdeI 143 CTAAG C^T[ACGT]AG
1734 CTCAG
FokI 21 GGATG GGATG(9/13)
363 GGATG
HapII 262 CCGG C^CGG
1766 CCGG
HpaII 262 CCGG C^CGG
1766 CCGG
MaeI 590 CTAG C^TAG
1409 CTAG
MaeIII 295 GTGAC ^GT[ACGT]AC
1712 GTGAC
MluNI 26 TGGCCA TGG^CCA
215 TGGCCA
MscI 26 TGGCCA TGG^CCA
215 TGGCCA
MslI 225 CACAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1526 CATACAAATG
MspI 262 CCGG C^CGG
1766 CCGG
Tsp45I 295 GTGAC ^GT[GC]AC
1712 GTGAC
TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^
299 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI AvaII BpmI
BsmAI BsrI BssKI BstNI Cfr13I
DpnII DraI EaeI EarI EcoRI
EcoRII HaeIII HindIII Ksp632I MboI
MboII MnlI MseI MvaI NdeII
NlaIII Sau3AI Sau96I ScrFI Tru9I
Tsp509I