##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BmyI        BpmI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CfoI        Cfr10I      ClaI        CpoI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI          628  GAATTT               [GA]^AATT[CT]
AlwNI         557  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          628  GAATTT               [GA]^AATT[CT]
Asp700I       580  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         163  GGTCC                G^G[AT]CC
BanI          460  GGCACC               G^G[CT][GA]CC
BbsI          532  GAAGAC               GAAGAC(2/6)
BlpI          118  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      118  GCTGAGC              GC^T[ACGT]AGC
BpuAI         532  GAAGAC               GAAGAC(2/6)
BsgI          563  CTGCAC               GTGCAG(16/14)
BsmFI         236  GGGAC                GGGAC(10/14)
BspMI         567  ACCTGC               ACCTGC(4/8)
BstAPI        556  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         644  AGATCC               [GA]^GATC[CT]
CelII         118  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        163  GGTCC                G^G[ACGT]CC
DraI          204  TTTAAA               TTT^AAA
DraII         162  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        506  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          311  CCACGG               C^C[GA][CT]GG
EarI           10  CTCTTC               CTCTTC(1/4)
EcoO109I      162  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        29  CCAAGG               C^C[AT][AT]GG
FauI          234  GCGGG                CCCGC(4/6)
HphI          511  GGTGA                GGTGA(8/7)
Ksp632I        10  CTCTTC               CTCTTC(1/4)
MflI          644  AGATCC               [GA]^GATC[CT]
MseI          205  TTAA                 T^TAA
MspA1I        232  CAGCGG               C[AC]G^C[GT]G
NlaIII        264  CATG                 CATG^
PpuMI         162  GGGTCCT              [GA]G^G[AT]CC[CT]
Sau96I        163  GGTCC                G^G[ACGT]CC
StyI           29  CCAAGG               C^C[AT][AT]GG
TfiI           85  GAATC                G^A[AT]TC
Tru9I         205  TTAA                 T^TAA
XhoII         644  AGATCC               [GA]^GATC[CT]
XmnI          580  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          645  GATCC                GGATC(4/5)
              138  GGATC               
BsaJI          29  CCAAGG               C^C[ACGT][ACGT]GG
              311  CCACGG              
DdeI          119  CTGAG                C^T[ACGT]AG
              445  CTGAG               
MwoI          452  GCAGTTATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              556  GCAGCATCTGC         
PleI            8  GACTC                GAGTC(4/5)
              447  GAGTC               
TaqI          108  TCGA                 T^CGA
              374  TCGA                
TspRI         443  CACTG                CA[GC]TG[ACGT][ACGT]^
              591  CACTG               
TthHB8I       108  TCGA                 T^CGA
              374  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BsiYI       
BslI        BsrI        BstF5I      DpnII       Fnu4HI      
FokI        HinfI       ItaI        MaeI        MboI        
MboII       MnlI        NdeII       NlaIV       Sau3AI      
SfaNI       TseI        Tsp509I