Restriction map for
pF1KB6984
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HapII HincII HindII HindIII HpaI
HpaII KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 364 CGCG CG^CG
AcyI 451 GGCGCC G[GA]^CG[CT]C
AflIII 545 ACATGT A^C[GA][CT]GT
AlwI 15 GATCC GGATC(4/5)
BanI 451 GGCGCC G^G[CT][GA]CC
BanII 444 GAGCTC G[GA]GC[CT]^C
BbeI 451 GGCGCC GGCGC^C
BbrPI 344 CACGTG CAC^GTG
BbsI 303 GAAGAC GAAGAC(2/6)
BlpI 495 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 495 GCTGAGC GC^T[ACGT]AGC
BpuAI 303 GAAGAC GAAGAC(2/6)
BsaAI 344 CACGTG [CT]AC^GT[GA]
BsaHI 451 GGCGCC G[GA]^CG[CT]C
BsgI 347 GTGCAG GTGCAG(16/14)
BspLU11I 545 ACATGT A^CATGT
BsrDI 617 GCAATG GCAATG(2/0)
BstAPI 407 GCACGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 364 CGCG CG^CG
BstXI 135 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 495 GCTGAGC GC^T[ACGT]AGC
CfoI 452 GCGC GCG^C
Cfr13I 604 GGCCC G^G[ACGT]CC
DraIII 408 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeII 451 GGCGCC [GA]GCGC^[CT]
HgaI 484 GACGC GACGC(5/10)
HhaI 452 GCGC GCG^C
Hin1I 451 GGCGCC G[GA]^CG[CT]C
HinP1I 452 GCGC G^CGC
KasI 451 GGCGCC G^GCGCC
MaeII 345 ACGT A^CGT
MspA1I 32 CAGCTG C[AC]G^C[GT]G
MvnI 364 CGCG CG^CG
NarI 451 GGCGCC GG^CGCC
NspI 545 ACATGT [GA]CATG^[CT]
PflMI 397 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 344 CACGTG CAC^GTG
PmlI 344 CACGTG CAC^GTG
PstI 472 CTGCAG CTGCA^G
PvuII 32 CAGCTG CAG^CTG
SacI 444 GAGCTC GAGCT^C
Sau96I 604 GGCCC G^G[ACGT]CC
SexAI 110 ACCAGGT A^CC[AT]GGT
SfaNI 203 GCATC GCATC(5/9)
SfcI 472 CTGCAG C^T[GA][CT]AG
SfoI 451 GGCGCC GGC^GCC
SmlI 565 CTTGAG C^T[CT][GA]AG
TaqI 233 TCGA T^CGA
TthHB8I 233 TCGA T^CGA
Van91I 397 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 135 CCACTTCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 682 GTCTAC GT^[AC][GT]AC
694 GTCTAC
AcsI 96 GAATTC [GA]^AATT[CT]
123 GAATTC
AlwNI 398 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
475 CAGTGCCTG
ApoI 96 GAATTC [GA]^AATT[CT]
123 GAATTC
AspHI 223 GAGCAC G[AT]GC[AT]^C
444 GAGCTC
BseRI 339 CTCCTC GAGGAG(10/8)
91 GAGGAG
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
444 GAGCTC
BsmAI 213 GAGAC GTCTC(1/5)
640 GTCTC
BsrI 216 ACTGG ACTGG(1/-1)
403 ACTGG
BstF5I 204 CATCC GGATG(2/0)
279 CATCC
DdeI 496 CTGAG C^T[ACGT]AG
506 CTAAG
EcoRI 96 GAATTC G^AATTC
123 GAATTC
EcoT14I 630 CCAAGG C^C[AT][AT]GG
689 CCAAGG
FauI 200 CCCGC CCCGC(4/6)
362 CCCGC
FokI 204 CATCC GGATG(9/13)
279 CATCC
HaeIII 184 GGCC GG^CC
604 GGCC
MboII 303 GAAGA GAAGA(8/7)
666 GAAGA
MseI 328 TTAA T^TAA
716 TTAA
MslI 136 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
204 CATCCAGGTG
NlaIV 148 GGCTCC GG[ACGT]^[ACGT]CC
451 GGCGCC
PleI 306 GACTC GAGTC(4/5)
680 GAGTC
StyI 630 CCAAGG C^C[AT][AT]GG
689 CCAAGG
Tru9I 328 TTAA T^TAA
716 TTAA
Tsp509I 97 AATT ^AATT
124 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I DpnII EcoRII
Fnu4HI HinfI HphI ItaI MboI
MnlI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI TfiI TseI TspRI