Restriction map for
pF1KB7810
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BfrI
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FspI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MslI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp45I
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 214 GTCTAC GT^[AC][GT]AC
AcsI 456 AAATTC [GA]^AATT[CT]
Alw44I 678 GTGCAC G^TGCAC
AlwNI 1504 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 678 GTGCAC G^TGCAC
ApoI 456 AAATTC [GA]^AATT[CT]
BbeI 169 GGCGCC GGCGC^C
BcgI 1082 CGAAGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 761 GGATAC GTATCC(6/5)
BglI 473 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1268 AGATCT A^GATCT
BlpI 964 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 964 GCTGAGC GC^T[ACGT]AGC
BsaI 1168 GGTCTC GGTCTC(1/5)
BsmBI 1684 GAGACG CGTCTC(1/5)
BsmFI 924 GTCCC GGGAC(10/14)
BspHI 887 TCATGA T^CATGA
BsrDI 563 GCAATG GCAATG(2/0)
BstXI 272 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 964 GCTGAGC GC^T[ACGT]AGC
DrdI 937 GACCAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 114 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 429 GGCCGGCC GGCCGG^CC
HindIII 1016 AAGCTT A^AGCTT
HphI 832 GGTGA GGTGA(8/7)
KasI 169 GGCGCC G^GCGCC
KspI 191 CCGCGG CCGC^GG
MaeII 1595 ACGT A^CGT
MaeIII 1553 GTAAC ^GT[ACGT]AC
MseI 1361 TTAA T^TAA
NarI 169 GGCGCC GG^CGCC
NcoI 1184 CCATGG C^CATGG
NotI 193 GCGGCCGC GC^GGCCGC
PvuII 346 CAGCTG CAG^CTG
RcaI 887 TCATGA T^CATGA
SacII 191 CCGCGG CCGC^GG
SapI 702 GAAGAGC GCTCTTC(1/4)
SfoI 169 GGCGCC GGC^GCC
SmaI 244 CCCGGG CCC^GGG
SmlI 91 CTCAAG C^T[CT][GA]AG
Tru9I 1361 TTAA T^TAA
XbaI 1134 TCTAGA T^CTAGA
XcmI 807 CCAGAGAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 244 CCCGGG C^CCGGG
XmaI 244 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 678 GTGCAC G[AT]GC[AT]^C
1189 GAGCAC
AvaI 163 CCCGAG C^[CT]CG[GA]G
244 CCCGGG
BsgI 331 CTGCAC GTGCAG(16/14)
554 GTGCAG
BsiEI 194 CGGCCG CG[GA][CT]^CG
932 CGGCCG
BsiHKAI 678 GTGCAC G[AT]GC[AT]^C
1189 GAGCAC
BsmI 442 GCATTC GAATGC(1/-1)
1728 GCATTC
BsoBI 163 CCCGAG C^[CT]CG[GA]G
244 CCCGGG
BsrBI 883 CCGCTC CCGCTC(-3/-3)
106 GAGCGG
BstAPI 1580 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1728 GCATTCCTTGC
EagI 194 CGGCCG C^GGCCG
932 CGGCCG
EclXI 194 CGGCCG C^GGCCG
932 CGGCCG
Eco52I 194 CGGCCG C^GGCCG
932 CGGCCG
HgaI 470 GACGC GACGC(5/10)
1633 GACGC
NspI 1277 ACATGC [GA]CATG^[CT]
1647 ACATGC
TfiI 529 GAATC G^A[AT]TC
1314 GAATC
Tsp509I 457 AATT ^AATT
1363 AATT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BalI BanI BanII
BbvI BclI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StuI StyI TaqI TseI
TspRI TthHB8I Van91I XhoII