Restriction map for
pF1KB6981
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 367 GTCTAC GT^[AC][GT]AC
AcsI 110 AAATTC [GA]^AATT[CT]
AcyI 637 GGCGTC G[GA]^CG[CT]C
AluI 236 AGCT AG^CT
AlwNI 304 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 251 AGCGCT AGC^GCT
ApoI 110 AAATTC [GA]^AATT[CT]
AvaI 345 CTCGGG C^[CT]CG[GA]G
AvaII 170 GGACC G^G[AT]CC
BalI 602 TGGCCA TGG^CCA
BanI 289 GGCACC G^G[CT][GA]CC
BbvCI 44 CCTCAGC CCTCAGC(-5/-2)
BcnI 285 CCGGG CC^[GC]GG
BglI 179 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 238 CTGGAG CTGGAG(16/14)
BsaHI 637 GGCGTC G[GA]^CG[CT]C
BsmI 585 GAATGC GAATGC(1/-1)
BsoBI 345 CTCGGG C^[CT]CG[GA]G
BspHI 482 TCATGA T^CATGA
BspMI 299 ACCTGC ACCTGC(4/8)
BsrI 36 ACTGG ACTGG(1/-1)
BssSI 520 CTCGTG CACGAG(-5/-1)
BstAPI 119 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 385 TTCGAA TT^CGAA
DraIII 162 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 563 CCACGG C^C[GA][CT]GG
EarI 379 CTCTTC CTCTTC(1/4)
Eco47III 251 AGCGCT AGC^GCT
FauI 53 GCGGG CCCGC(4/6)
HapII 285 CCGG C^CGG
Hin1I 637 GGCGTC G[GA]^CG[CT]C
HincII 507 GTTGAC GT[CT]^[GA]AC
HindII 507 GTTGAC GT[CT]^[GA]AC
HinfI 73 GACTC G^A[ACGT]TC
HpaII 285 CCGG C^CGG
Ksp632I 379 CTCTTC CTCTTC(1/4)
MaeII 365 ACGT A^CGT
MaeIII 331 GTCAC ^GT[ACGT]AC
MluNI 602 TGGCCA TGG^CCA
MscI 602 TGGCCA TGG^CCA
MslI 498 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 235 CAGCTG C[AC]G^C[GT]G
MspI 285 CCGG C^CGG
NciI 285 CCGGG CC^[GC]GG
NdeI 513 CATATG CA^TATG
NlaIV 289 GGCACC GG[ACGT]^[ACGT]CC
NspV 385 TTCGAA TT^CGAA
PleI 73 GACTC GAGTC(4/5)
PstI 301 CTGCAG CTGCA^G
PvuII 235 CAGCTG CAG^CTG
RcaI 482 TCATGA T^CATGA
SapI 378 GCTCTTC GCTCTTC(1/4)
SbfI 300 CCTGCAGG CCTGCA^GG
SfcI 301 CTGCAG C^T[GA][CT]AG
SfuI 385 TTCGAA TT^CGAA
Sse8387I 300 CCTGCAGG CCTGCA^GG
Tsp45I 331 GTCAC ^GT[GC]AC
Tsp509I 111 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 30 CGCG CG^CG
265 CGCG
AspHI 397 GAGCAC G[AT]GC[AT]^C
493 GTGCTC
BseRI 193 GAGGAG GAGGAG(10/8)
241 GAGGAG
BsgI 575 CTGCAC GTGCAG(16/14)
630 CTGCAC
BsiHKAI 397 GAGCAC G[AT]GC[AT]^C
493 GTGCTC
BstNI 600 CCTGG CC^[AT]GG
642 CCAGG
BstUI 30 CGCG CG^CG
265 CGCG
Cfr13I 170 GGACC G^G[ACGT]CC
261 GGGCC
EaeI 602 TGGCCA [CT]^GGCC[GA]
647 TGGCCG
EcoRII 600 CCTGG ^CC[AT]GG
642 CCAGG
HaeII 131 AGCGCC [GA]GCGC^[CT]
251 AGCGCT
HgaI 410 GCGTC GACGC(5/10)
638 GCGTC
MvaI 600 CCTGG CC^[AT]GG
642 CCAGG
MvnI 30 CGCG CG^CG
265 CGCG
NlaIII 459 CATG CATG^
483 CATG
Sau96I 170 GGACC G^G[ACGT]CC
261 GGGCC
SfaNI 119 GCATC GCATC(5/9)
463 GCATC
TaqI 257 TCGA T^CGA
386 TCGA
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
189 CACTG
TthHB8I 257 TCGA T^CGA
386 TCGA
XcmI 449 CCAACAGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
512 CCATATGGCTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AlwI BbvI BmyI BsaI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstYI Cac8I CfoI
DdeI DpnII Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MboI
MboII MflI MnlI MwoI NdeII
Sau3AI ScrFI TseI XhoII