Restriction map for
pF1KB6973
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboII MfeI MluI MluNI
MroI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 186 GAATTT [GA]^AATT[CT]
AlwNI 208 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 532 GGGCCC GGGCC^C
ApoI 186 GAATTT [GA]^AATT[CT]
BanI 633 GGCGCC G^G[CT][GA]CC
BanII 532 GGGCCC G[GA]GC[CT]^C
BbeI 633 GGCGCC GGCGC^C
BglII 365 AGATCT A^GATCT
BlpI 456 GCTGAGC GC^T[ACGT]AGC
BmyI 532 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BseRI 520 GAGGAG GAGGAG(10/8)
BsgI 369 CTGCAC GTGCAG(16/14)
BsiEI 620 CGGCCG CG[GA][CT]^CG
BsmAI 478 GAGAC GTCTC(1/5)
BsmBI 478 GAGACG CGTCTC(1/5)
Bsp1286I 532 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 305 TGTACA T^GTACA
BspMI 334 ACCTGC ACCTGC(4/8)
BsrFI 483 GCCGGC [GA]^CCGG[CT]
BsrGI 305 TGTACA T^GTACA
BstYI 365 AGATCT [GA]^GATC[CT]
CelII 456 GCTGAGC GC^T[ACGT]AGC
Cfr10I 483 GCCGGC [GA]^CCGG[CT]
DdeI 457 CTGAG C^T[ACGT]AG
DrdI 130 GACCAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 620 CGGCCG C^GGCCG
EclXI 620 CGGCCG C^GGCCG
Eco52I 620 CGGCCG C^GGCCG
HgaI 480 GACGC GACGC(5/10)
KasI 633 GGCGCC G^GCGCC
MflI 365 AGATCT [GA]^GATC[CT]
MslI 399 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 483 GCCGGC GCC^GGC
NarI 633 GGCGCC GG^CGCC
NgoMIV 483 GCCGGC G^CCGGC
PvuII 163 CAGCTG CAG^CTG
SexAI 131 ACCAGGT A^CC[AT]GGT
SfaNI 428 GCATC GCATC(5/9)
SfiI 438 GGCCATCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 633 GGCGCC GGC^GCC
SgrAI 482 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 574 CCCGGG CCC^GGG
SspBI 305 TGTACA T^GTACA
Tsp509I 187 AATT ^AATT
TspRI 75 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 275 CCATGGCGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 365 AGATCT [GA]^GATC[CT]
XmaCI 574 CCCGGG C^CCGGG
XmaI 574 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 480 GACGCC G[GA]^CG[CT]C
633 GGCGCC
AvaI 412 CCCGAG C^[CT]CG[GA]G
574 CCCGGG
AvaII 51 GGACC G^G[AT]CC
408 GGACC
BclI 86 TGATCA T^GATCA
299 TGATCA
BglI 338 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
439 GCCATCGGGGC
BsaHI 480 GACGCC G[GA]^CG[CT]C
633 GGCGCC
BsmFI 50 GGGAC GGGAC(10/14)
497 GGGAC
BsoBI 412 CCCGAG C^[CT]CG[GA]G
574 CCCGGG
BsrI 285 ACTGG ACTGG(1/-1)
327 CCAGT
DraII 445 GGGGCCT [GA]G^G[ACGT]CC[CT]
531 GGGGCCC
DsaI 68 CCATGG C^C[GA][CT]GG
275 CCATGG
EcoO109I 445 GGGGCCT [GA]G^G[ACGT]CC[CT]
531 GGGGCCC
FbaI 86 TGATCA T^GATCA
299 TGATCA
Hin1I 480 GACGCC G[GA]^CG[CT]C
633 GGCGCC
HphI 3 GGTGA GGTGA(8/7)
332 TCACC
NcoI 68 CCATGG C^CATGG
275 CCATGG
PstI 15 CTGCAG CTGCA^G
219 CTGCAG
SfcI 15 CTGCAG C^T[GA][CT]AG
219 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BcnI BpmI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI StyI TseI