Restriction map for
pF1KB8844
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BbsI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MroI MslI MunI
NheI NotI NruI NspI NspV
PacI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1054 GACGTC GACGT^C
AocI 1368 CCTCAGG CC^T[ACGT]AGG
ApaI 1153 GGGCCC GGGCC^C
Asp700I 1350 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 658 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 734 TGGCCA TGG^CCA
BamHI 893 GGATCC G^GATCC
BbeI 177 GGCGCC GGCGC^C
BbrPI 273 CACGTG CAC^GTG
BbvCI 39 GCTGAGG CCTCAGC(-5/-2)
BciVI 577 GGATAC GTATCC(6/5)
BclI 1505 TGATCA T^GATCA
BfaI 973 CTAG C^TAG
BpmI 772 CTGGAG CTGGAG(16/14)
BsaBI 289 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 42 GAGGAG GAGGAG(10/8)
BsiEI 1127 CGGCCG CG[GA][CT]^CG
BsmI 1555 GAATGC GAATGC(1/-1)
BstAPI 846 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1368 CCTCAGG CC^T[ACGT]AGG
DraIII 1276 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1127 CGGCCG C^GGCCG
EclXI 1127 CGGCCG C^GGCCG
Eco52I 1127 CGGCCG C^GGCCG
Eco81I 1368 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1295 CCATGG C^C[AT][AT]GG
EcoT22I 1429 ATGCAT ATGCA^T
FbaI 1505 TGATCA T^GATCA
KasI 177 GGCGCC G^GCGCC
MaeI 973 CTAG C^TAG
MamI 289 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
NarI 177 GGCGCC GG^CGCC
NcoI 1295 CCATGG C^CATGG
NdeI 499 CATATG CA^TATG
NsiI 1429 ATGCAT ATGCA^T
PaeR7I 646 CTCGAG C^TCGAG
PflFI 658 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1275 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 273 CACGTG CAC^GTG
PmlI 273 CACGTG CAC^GTG
SapI 1234 GCTCTTC GCTCTTC(1/4)
SfoI 177 GGCGCC GGC^GCC
StyI 1295 CCATGG C^C[AT][AT]GG
Tth111I 658 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1275 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1427 CCATGCATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 646 CTCGAG C^TCGAG
XmnI 1350 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 962 ACGTGT A^C[GA][CT]GT
1340 ACGTGT
AvaI 51 CTCGGG C^[CT]CG[GA]G
646 CTCGAG
AvaII 426 GGACC G^G[AT]CC
1326 GGTCC
AviII 416 TGCGCA TGC^GCA
533 TGCGCA
BcgI 605 GCAACTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
794 GCAACGAGATCG
BsaAI 273 CACGTG [CT]AC^GT[GA]
1339 TACGTG
BsaI 829 GAGACC GGTCTC(1/5)
19 GGTCTC
BsmAI 829 GAGAC GTCTC(1/5)
20 GTCTC
BsoBI 51 CTCGGG C^[CT]CG[GA]G
646 CTCGAG
BssHII 240 GCGCGC G^CGCGC
601 GCGCGC
BssSI 978 CTCGTG CACGAG(-5/-1)
1524 CTCGTG
BstF5I 516 CATCC GGATG(2/0)
1092 GGATG
BstYI 893 GGATCC [GA]^GATC[CT]
1271 AGATCC
EarI 466 CTCTTC CTCTTC(1/4)
1235 CTCTTC
EcoNI 168 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1083 CCTGTGCGAGG
FokI 516 CATCC GGATG(9/13)
1092 GGATG
FspI 416 TGCGCA TGC^GCA
533 TGCGCA
HgaI 319 GACGC GACGC(5/10)
1063 GACGC
HinfI 594 GAGTC G^A[ACGT]TC
862 GACTC
Ksp632I 466 CTCTTC CTCTTC(1/4)
1235 CTCTTC
MflI 893 GGATCC [GA]^GATC[CT]
1271 AGATCC
NaeI 244 GCCGGC GCC^GGC
616 GCCGGC
NgoMIV 244 GCCGGC G^CCGGC
616 GCCGGC
PleI 862 GACTC GAGTC(4/5)
594 GAGTC
PvuII 121 CAGCTG CAG^CTG
856 CAGCTG
XhoII 893 GGATCC [GA]^GATC[CT]
1271 AGATCC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI BanI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I