Restriction map for
pF1KB6967
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
Acc65I AccI AclI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI KspI MaeI MaeIII
MfeI MflI MluI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I XbaI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 578 GACGTC GACGT^C
AccII 336 CGCG CG^CG
AccIII 224 TCCGGA T^CCGGA
AcsI 383 AAATTT [GA]^AATT[CT]
AcyI 578 GACGTC G[GA]^CG[CT]C
AlwNI 65 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 383 AAATTT [GA]^AATT[CT]
Asp700I 380 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 16 CTCGAG C^[CT]CG[GA]G
BalI 429 TGGCCA TGG^CCA
BanI 40 GGCACC G^G[CT][GA]CC
BbsI 87 GAAGAC GAAGAC(2/6)
BpuAI 87 GAAGAC GAAGAC(2/6)
BsaHI 578 GACGTC G[GA]^CG[CT]C
BsaWI 224 TCCGGA [AT]^CCGG[AT]
BseAI 224 TCCGGA T^CCGGA
BseRI 488 CTCCTC GAGGAG(10/8)
BsgI 623 GTGCAG GTGCAG(16/14)
BsoBI 16 CTCGAG C^[CT]CG[GA]G
BspEI 224 TCCGGA T^CCGGA
BssSI 528 CTCGTG CACGAG(-5/-1)
BstUI 336 CGCG CG^CG
DdeI 277 CTGAG C^T[ACGT]AG
DraI 507 TTTAAA TTT^AAA
EaeI 429 TGGCCA [CT]^GGCC[GA]
FauI 337 GCGGG CCCGC(4/6)
HapII 225 CCGG C^CGG
HgaI 325 GCGTC GACGC(5/10)
Hin1I 578 GACGTC G[GA]^CG[CT]C
HincII 11 GTCAAC GT[CT]^[GA]AC
HindII 11 GTCAAC GT[CT]^[GA]AC
HpaII 225 CCGG C^CGG
HphI 388 TCACC GGTGA(8/7)
MaeII 579 ACGT A^CGT
MluNI 429 TGGCCA TGG^CCA
MroI 224 TCCGGA T^CCGGA
MscI 429 TGGCCA TGG^CCA
MseI 508 TTAA T^TAA
MslI 232 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 225 CCGG C^CGG
MvnI 336 CGCG CG^CG
PaeR7I 16 CTCGAG C^TCGAG
PflMI 64 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 188 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 590 GAAGAGC GCTCTTC(1/4)
SmlI 16 CTCGAG C^T[CT][GA]AG
TaqI 17 TCGA T^CGA
Tru9I 508 TTAA T^TAA
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 17 TCGA T^CGA
Van91I 64 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 171 CCACCAAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 16 CTCGAG C^TCGAG
XmnI 380 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 297 GCGG CCGC(-3/-1)
337 GCGG
AspHI 262 GAGCTC G[AT]GC[AT]^C
281 GAGCTC
AvaII 27 GGACC G^G[AT]CC
54 GGACC
BanII 262 GAGCTC G[GA]GC[CT]^C
281 GAGCTC
BsaBI 467 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
559 GATAAAAATC
BsiHKAI 262 GAGCTC G[AT]GC[AT]^C
281 GAGCTC
BsmBI 576 GAGACG CGTCTC(1/5)
630 GAGACG
BspMI 369 ACCTGC ACCTGC(4/8)
7 GCAGGT
BsrI 130 ACTGG ACTGG(1/-1)
604 ACTGG
BstXI 174 CCAAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
449 CCACTCTCCTGG
Cac8I 3 GCTGGC GC[ACGT]^[ACGT]GC
427 GCTGGC
Cfr13I 27 GGACC G^G[ACGT]CC
54 GGACC
DpnII 159 GATC ^GATC
467 GATC
DraII 26 GGGACCC [GA]G^G[ACGT]CC[CT]
53 AGGACCC
EarI 406 GAAGAG CTCTTC(1/4)
590 GAAGAG
EcoO109I 26 GGGACCC [GA]G^G[ACGT]CC[CT]
53 AGGACCC
Ksp632I 406 GAAGAG CTCTTC(1/4)
590 GAAGAG
MamI 467 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
559 GATAAAAATC
MboI 159 GATC ^GATC
467 GATC
NdeII 159 GATC ^GATC
467 GATC
PleI 167 GACTC GAGTC(4/5)
228 GAGTC
PpuMI 26 GGGACCC [GA]G^G[AT]CC[CT]
53 AGGACCC
SacI 262 GAGCTC GAGCT^C
281 GAGCTC
Sau3AI 159 GATC ^GATC
467 GATC
Sau96I 27 GGACC G^G[ACGT]CC
54 GGACC
SfaNI 138 GATGC GCATC(5/9)
238 GATGC
TfiI 346 GAATC G^A[AT]TC
438 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI EcoRII Fnu4HI HaeIII HinfI
ItaI MboII MnlI MspA1I MvaI
MwoI NlaIII NlaIV PstI PvuII
ScrFI SfcI TseI Tsp509I