Restriction map for
pF1KB6966
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 275 GGTACC G^GTACC
Alw44I 217 GTGCAC G^TGCAC
ApaLI 217 GTGCAC G^TGCAC
Asp718I 275 GGTACC G^GTACC
AvaII 152 GGACC G^G[AT]CC
BanI 275 GGTACC G^G[CT][GA]CC
BseRI 679 GAGGAG GAGGAG(10/8)
BsgI 163 GTGCAG GTGCAG(16/14)
BsmFI 393 GGGAC GGGAC(10/14)
BspMI 18 GCAGGT ACCTGC(4/8)
BsrDI 632 GCAATG GCAATG(2/0)
BsrI 464 CCAGT ACTGG(1/-1)
CpoI 151 CGGACCG CG^G[AT]CCG
DpnII 684 GATC ^GATC
DraII 713 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 713 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 136 GTTGAC GT[CT]^[GA]AC
HindII 136 GTTGAC GT[CT]^[GA]AC
KpnI 275 GGTACC GGTAC^C
MboI 684 GATC ^GATC
MseI 430 TTAA T^TAA
MslI 210 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 314 CAGCTG C[AC]G^C[GT]G
NdeII 684 GATC ^GATC
NlaIII 93 CATG CATG^
PvuII 314 CAGCTG CAG^CTG
RsrII 151 CGGACCG CG^G[AT]CCG
Sau3AI 684 GATC ^GATC
SfaNI 618 GCATC GCATC(5/9)
SmaI 644 CCCGGG CCC^GGG
StuI 602 AGGCCT AGG^CCT
TfiI 587 GATTC G^A[AT]TC
Tru9I 430 TTAA T^TAA
Tsp45I 115 GTCAC ^GT[GC]AC
XmaCI 644 CCCGGG C^CCGGG
XmaI 644 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 16 CCGC CCGC(-3/-1)
150 GCGG
AlwNI 82 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
166 CAGAAGCTG
AvaI 598 CCCGAG C^[CT]CG[GA]G
644 CCCGGG
BanII 514 GAGCTC G[GA]GC[CT]^C
688 GAGCTC
BbvI 186 GCAGC GCAGC(8/12)
289 GCAGC
BpmI 250 CTGGAG CTGGAG(16/14)
385 CTGGAG
BsoBI 598 CCCGAG C^[CT]CG[GA]G
644 CCCGGG
DdeI 302 CTGAG C^T[ACGT]AG
488 CTCAG
EcoRI 357 GAATTC G^AATTC
652 GAATTC
Fnu4HI 186 GCAGC GC^[ACGT]GC
289 GCAGC
ItaI 186 GCAGC GC^[ACGT]GC
289 GCAGC
NlaIV 52 GGGGCC GG[ACGT]^[ACGT]CC
275 GGTACC
SacI 514 GAGCTC GAGCT^C
688 GAGCTC
SmlI 106 CTCAAG C^T[CT][GA]AG
627 CTTGAG
TaqI 23 TCGA T^CGA
686 TCGA
TseI 186 GCAGC G^C[AT]GC
289 GCAGC
TthHB8I 23 TCGA T^CGA
686 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII FokI
HaeIII HapII HinfI HpaII HphI
MaeIII MboII MnlI MspI MvaI
NciI PleI RsaI Sau96I ScrFI
Tsp509I TspRI