Restriction map for
pF1KB9870
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 30 GTAC GT^AC
AlwI 765 GATCC GGATC(4/5)
ApaI 109 GGGCCC GGGCC^C
Asp700I 1419 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 33 CCCGGG C^[CT]CG[GA]G
AviII 184 TGCGCA TGC^GCA
BalI 590 TGGCCA TGG^CCA
BcgI 630 CGACGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 899 TGATCA T^GATCA
BglII 1316 AGATCT A^GATCT
BsaWI 522 TCCGGT [AT]^CCGG[AT]
BsmBI 1153 GAGACG CGTCTC(1/5)
BsmI 636 GAATGC GAATGC(1/-1)
BsoBI 33 CCCGGG C^[CT]CG[GA]G
BspHI 719 TCATGA T^CATGA
BsrDI 1169 GCAATG GCAATG(2/0)
DrdI 1024 GACGTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 77 CCGTGG C^C[GA][CT]GG
EagI 989 CGGCCG C^GGCCG
EarI 681 CTCTTC CTCTTC(1/4)
EclXI 989 CGGCCG C^GGCCG
Eco52I 989 CGGCCG C^GGCCG
FbaI 899 TGATCA T^GATCA
FspI 184 TGCGCA TGC^GCA
HaeII 270 GGCGCT [GA]GCGC^[CT]
Ksp632I 681 CTCTTC CTCTTC(1/4)
MluNI 590 TGGCCA TGG^CCA
MscI 590 TGGCCA TGG^CCA
MseI 944 TTAA T^TAA
NaeI 991 GCCGGC GCC^GGC
NgoMIV 991 GCCGGC G^CCGGC
NspI 1307 GCATGT [GA]CATG^[CT]
PpuMI 1293 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 225 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 963 CTGCAG CTGCA^G
PvuI 1302 CGATCG CGAT^CG
RcaI 719 TCATGA T^CATGA
RsaI 30 GTAC GT^AC
SacI 1174 GAGCTC GAGCT^C
SfcI 963 CTGCAG C^T[GA][CT]AG
SgrAI 1145 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 33 CCCGGG CCC^GGG
SmlI 883 CTCAAG C^T[CT][GA]AG
Tru9I 944 TTAA T^TAA
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
XmnI 1419 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 55 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1111 CAGCTGCTG
BanI 22 GGCACC G^G[CT][GA]CC
96 GGCACC
BglI 102 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
301 GCCGAGGTGGC
BsaI 871 GAGACC GGTCTC(1/5)
1012 GAGACC
BsgI 760 GTGCAG GTGCAG(16/14)
1122 GTGCAG
BsiEI 989 CGGCCG CG[GA][CT]^CG
1302 CGATCG
BspMI 209 ACCTGC ACCTGC(4/8)
575 ACCTGC
BstEII 123 GGTCACC G^GT[ACGT]ACC
1339 GGTGACC
BstPI 123 GGTCACC G^GT[ACGT]ACC
1339 GGTGACC
BstYI 764 AGATCC [GA]^GATC[CT]
1316 AGATCT
DdeI 458 CTCAG C^T[ACGT]AG
1352 CTGAG
EcoO65I 123 GGTCACC G^GT[ACGT]ACC
1339 GGTGACC
EcoT14I 470 CCTTGG C^C[AT][AT]GG
875 CCAAGG
MflI 764 AGATCC [GA]^GATC[CT]
1316 AGATCT
PflMI 54 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1110 CCAGCTGCTGG
StyI 470 CCTTGG C^C[AT][AT]GG
875 CCAAGG
TfiI 663 GAATC G^A[AT]TC
1424 GATTC
Van91I 54 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1110 CCAGCTGCTGG
XhoII 764 AGATCC [GA]^GATC[CT]
1316 AGATCT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AspHI AspI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I