Restriction map for
pF1KB6951
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 144 GACGCC G[GA]^CG[CT]C
Aor51HI 382 AGCGCT AGC^GCT
AvaII 93 GGACC G^G[AT]CC
BamHI 464 GGATCC G^GATCC
BclI 248 TGATCA T^GATCA
BpmI 631 CTGGAG CTGGAG(16/14)
BsaAI 495 CACGTA [CT]AC^GT[GA]
BsaHI 144 GACGCC G[GA]^CG[CT]C
BsaWI 150 TCCGGT [AT]^CCGG[AT]
BsmAI 142 GAGAC GTCTC(1/5)
BsmBI 142 GAGACG CGTCTC(1/5)
BsmFI 132 GTCCC GGGAC(10/14)
BsmI 225 GCATTC GAATGC(1/-1)
BspLU11I 534 ACATGT A^CATGT
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BstNI 620 CCAGG CC^[AT]GG
BstXI 444 CCATAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 383 GCGC GCG^C
DsaI 135 CCGTGG C^C[GA][CT]GG
Eco47III 382 AGCGCT AGC^GCT
EcoRII 620 CCAGG ^CC[AT]GG
EcoT22I 24 ATGCAT ATGCA^T
FauI 30 CCCGC CCCGC(4/6)
FbaI 248 TGATCA T^GATCA
HaeII 382 AGCGCT [GA]GCGC^[CT]
HgaI 144 GACGC GACGC(5/10)
HhaI 383 GCGC GCG^C
Hin1I 144 GACGCC G[GA]^CG[CT]C
HinP1I 383 GCGC G^CGC
MaeII 496 ACGT A^CGT
MboII 45 GAAGA GAAGA(8/7)
MspA1I 628 CAGCTG C[AC]G^C[GT]G
MvaI 620 CCAGG CC^[AT]GG
NlaIV 464 GGATCC GG[ACGT]^[ACGT]CC
NsiI 24 ATGCAT ATGCA^T
PleI 173 GAGTC GAGTC(4/5)
PpuMI 92 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 628 CAGCTG CAG^CTG
SmlI 412 CTCAAG C^T[CT][GA]AG
TaqI 350 TCGA T^CGA
TfiI 291 GAATC G^A[AT]TC
Tsp45I 440 GTGAC ^GT[GC]AC
Tsp509I 573 AATT ^AATT
TthHB8I 350 TCGA T^CGA
XcmI 620 CCAGGGTTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 494 ACACGT A^C[GA][CT]GT
534 ACATGT
BcnI 189 CCGGG CC^[GC]GG
461 CCCGG
BglII 303 AGATCT A^GATCT
521 AGATCT
BseRI 262 GAGGAG GAGGAG(10/8)
343 GAGGAG
BsgI 82 GTGCAG GTGCAG(16/14)
257 GTGCAG
BsrI 102 CCAGT ACTGG(1/-1)
483 CCAGT
DraII 92 AGGACCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCT
EcoO109I 92 AGGACCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCT
HinfI 173 GAGTC G^A[ACGT]TC
291 GAATC
HphI 153 GGTGA GGTGA(8/7)
288 GGTGA
MseI 214 TTAA T^TAA
311 TTAA
MslI 75 CATTAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
445 CATAATGGTG
NciI 189 CCGGG CC^[GC]GG
461 CCCGG
NspI 22 ACATGC [GA]CATG^[CT]
534 ACATGT
Tru9I 214 TTAA T^TAA
311 TTAA
TspRI 103 CAGTG CA[GC]TG[ACGT][ACGT]^
596 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeIII MboI MflI MnlI MspI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI TseI XhoII