Restriction map for
pF1KB6950
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I Cac8I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 10 CGCG CG^CG
Alw44I 259 GTGCAC G^TGCAC
AlwI 448 GATCC GGATC(4/5)
ApaI 237 GGGCCC GGGCC^C
ApaLI 259 GTGCAC G^TGCAC
BbsI 510 GAAGAC GAAGAC(2/6)
BcgI 261 GCACAGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 277 CTAG C^TAG
BglII 540 AGATCT A^GATCT
BlpI 322 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 322 GCTCAGC GC^T[ACGT]AGC
BpuAI 510 GAAGAC GAAGAC(2/6)
BsaJI 104 CCGTGG C^C[ACGT][ACGT]GG
BseRI 92 GAGGAG GAGGAG(10/8)
BsmFI 423 GGGAC GGGAC(10/14)
BssKI 470 CCTGG ^CC[ACGT]GG
BstNI 470 CCTGG CC^[AT]GG
BstUI 10 CGCG CG^CG
CelII 322 GCTCAGC GC^T[ACGT]AGC
CfoI 9 GCGC GCG^C
DraII 237 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 104 CCGTGG C^C[GA][CT]GG
EcoO109I 237 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 470 CCTGG ^CC[AT]GG
FauI 11 GCGGG CCCGC(4/6)
HaeIII 238 GGCC GG^CC
HhaI 9 GCGC GCG^C
HinP1I 9 GCGC G^CGC
HindIII 591 AAGCTT A^AGCTT
HinfI 632 GAATC G^A[ACGT]TC
MaeI 277 CTAG C^TAG
MspA1I 325 CAGCTG C[AC]G^C[GT]G
MvaI 470 CCTGG CC^[AT]GG
MvnI 10 CGCG CG^CG
NlaIII 234 CATG CATG^
PvuII 325 CAGCTG CAG^CTG
SacI 266 GAGCTC GAGCT^C
ScrFI 470 CCTGG CC^[ACGT]GG
SmlI 520 CTTGAG C^T[CT][GA]AG
TaqI 270 TCGA T^CGA
TfiI 632 GAATC G^A[AT]TC
TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 270 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
11 GCGG
Asp700I 307 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
510 GAAGACTTTC
AspHI 259 GTGCAC G[AT]GC[AT]^C
266 GAGCTC
BpmI 89 CTGGAG CTGGAG(16/14)
455 CTGGAG
BsiHKAI 259 GTGCAC G[AT]GC[AT]^C
266 GAGCTC
BsiYI 99 CCCGTCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
100 CCGTCCGTGGG
BslI 99 CCCGTCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
100 CCGTCCGTGGG
Bsp1407I 183 TGTACA T^GTACA
224 TGTACA
BsrDI 484 GCAATG GCAATG(2/0)
638 GCAATG
BsrGI 183 TGTACA T^GTACA
224 TGTACA
BstYI 447 AGATCC [GA]^GATC[CT]
540 AGATCT
Cfr13I 237 GGGCC G^G[ACGT]CC
238 GGCCC
DpnII 448 GATC ^GATC
541 GATC
MboI 448 GATC ^GATC
541 GATC
MflI 447 AGATCC [GA]^GATC[CT]
540 AGATCT
NdeII 448 GATC ^GATC
541 GATC
Sau3AI 448 GATC ^GATC
541 GATC
Sau96I 237 GGGCC G^G[ACGT]CC
238 GGCCC
SfaNI 351 GATGC GCATC(5/9)
556 GATGC
SspBI 183 TGTACA T^GTACA
224 TGTACA
Tsp509I 111 AATT ^AATT
201 AATT
XhoII 447 AGATCC [GA]^GATC[CT]
540 AGATCT
XmnI 307 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
510 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
Bsp1286I DdeI Fnu4HI ItaI MboII
MnlI MseI MwoI NlaIV RsaI
SfcI Tru9I TseI