##################################
# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AlwI 114 GATCC GGATC(4/5)
AlwNI 297 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 233 AGCGCT AGC^GCT
AvaII 675 GGACC G^G[AT]CC
BciVI 16 GGATAC GTATCC(6/5)
BmyI 273 GGGCAC G[AGT]GC[ACT]^C
BpmI 315 CTGGAG CTGGAG(16/14)
BsaJI 668 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 425 ACCGGA [AT]^CCGG[AT]
BseRI 78 CTCCTC GAGGAG(10/8)
BsgI 387 CTGCAC GTGCAG(16/14)
BsmAI 58 GTCTC GTCTC(1/5)
BsmBI 57 CGTCTC CGTCTC(1/5)
BsmI 399 GAATGC GAATGC(1/-1)
Bsp1286I 273 GGGCAC G[AGT]GC[ACT]^C
BsrI 314 ACTGG ACTGG(1/-1)
Cac8I 658 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 534 CTCAG C^T[ACGT]AG
DpnII 114 GATC ^GATC
EarI 370 GAAGAG CTCTTC(1/4)
Eco47III 233 AGCGCT AGC^GCT
EcoT14I 668 CCAAGG C^C[AT][AT]GG
HaeII 233 AGCGCT [GA]GCGC^[CT]
HaeIII 67 GGCC GG^CC
HapII 426 CCGG C^CGG
HpaII 426 CCGG C^CGG
HphI 23 TCACC GGTGA(8/7)
Ksp632I 370 GAAGAG CTCTTC(1/4)
MaeIII 305 GTGAC ^GT[ACGT]AC
MboI 114 GATC ^GATC
MslI 390 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 426 CCGG C^CGG
NdeII 114 GATC ^GATC
PleI 456 GACTC GAGTC(4/5)
Sau3AI 114 GATC ^GATC
ScaI 383 AGTACT AGT^ACT
SfaNI 73 GCATC GCATC(5/9)
SspI 31 AATATT AAT^ATT
StyI 668 CCAAGG C^C[AT][AT]GG
TaqI 496 TCGA T^CGA
TfiI 105 GATTC G^A[AT]TC
Tsp45I 305 GTGAC ^GT[GC]AC
TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 496 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 384 GTAC GT^AC
441 GTAC
AluI 51 AGCT AG^CT
334 AGCT
BssKI 83 CCTGG ^CC[ACGT]GG
452 CCAGG
BstNI 83 CCTGG CC^[AT]GG
452 CCAGG
CfoI 174 GCGC GCG^C
234 GCGC
Cfr13I 67 GGCCC G^G[ACGT]CC
675 GGACC
EcoRII 83 CCTGG ^CC[AT]GG
452 CCAGG
HhaI 174 GCGC GCG^C
234 GCGC
HinP1I 174 GCGC G^CGC
234 GCGC
HinfI 105 GATTC G^A[ACGT]TC
456 GACTC
MvaI 83 CCTGG CC^[AT]GG
452 CCAGG
RsaI 384 GTAC GT^AC
441 GTAC
Sau96I 67 GGCCC G^G[ACGT]CC
675 GGACC
ScrFI 83 CCTGG CC^[ACGT]GG
452 CCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BsiYI BslI BstF5I Fnu4HI
FokI ItaI MboII MnlI MseI
NlaIII Tru9I TseI Tsp509I