Restriction map for
pF1KB6923
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 40 CGCG CG^CG
AcsI 599 AAATTC [GA]^AATT[CT]
AfaI 487 GTAC GT^AC
Alw44I 341 GTGCAC G^TGCAC
AlwNI 244 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 341 GTGCAC G^TGCAC
ApoI 599 AAATTC [GA]^AATT[CT]
BanII 178 GAGCTC G[GA]GC[CT]^C
BclI 392 TGATCA T^GATCA
BsaJI 17 CCCGGG C^C[ACGT][ACGT]GG
BsgI 136 GTGCAG GTGCAG(16/14)
BsmAI 357 GTCTC GTCTC(1/5)
BsmFI 510 GGGAC GGGAC(10/14)
BsrI 249 ACTGG ACTGG(1/-1)
BstF5I 375 GGATG GGATG(2/0)
BstUI 40 CGCG CG^CG
DraII 284 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 59 TGGCCG [CT]^GGCC[GA]
EarI 502 GAAGAG CTCTTC(1/4)
EcoO109I 284 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 41 GCGGG CCCGC(4/6)
FbaI 392 TGATCA T^GATCA
FokI 375 GGATG GGATG(9/13)
HapII 18 CCGG C^CGG
HpaII 18 CCGG C^CGG
Ksp632I 502 GAAGAG CTCTTC(1/4)
MspI 18 CCGG C^CGG
MvnI 40 CGCG CG^CG
MwoI 80 GCATCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 585 CATG CATG^
NlaIV 510 GGGACC GG[ACGT]^[ACGT]CC
PaeR7I 131 CTCGAG C^TCGAG
PpuMI 284 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 7 CTGCAG CTGCA^G
RsaI 487 GTAC GT^AC
SacI 178 GAGCTC GAGCT^C
SfaNI 80 GCATC GCATC(5/9)
SfcI 7 CTGCAG C^T[GA][CT]AG
SmaI 17 CCCGGG CCC^GGG
SspI 268 AATATT AAT^ATT
TaqI 132 TCGA T^CGA
TfiI 29 GAATC G^A[AT]TC
TthHB8I 132 TCGA T^CGA
XhoI 131 CTCGAG C^TCGAG
XmaCI 17 CCCGGG C^CCGGG
XmaI 17 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 543 CCGC CCGC(-3/-1)
41 GCGG
AspHI 178 GAGCTC G[AT]GC[AT]^C
341 GTGCAC
AvaI 17 CCCGGG C^[CT]CG[GA]G
131 CTCGAG
AvaII 285 GGTCC G^G[AT]CC
511 GGACC
BbvI 9 GCAGC GCAGC(8/12)
6 GCTGC
BcnI 17 CCCGG CC^[GC]GG
18 CCGGG
BmyI 178 GAGCTC G[AGT]GC[ACT]^C
341 GTGCAC
BsiHKAI 178 GAGCTC G[AT]GC[AT]^C
341 GTGCAC
BsiYI 18 CCGGGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
35 CCCTGCGCGGG
BslI 18 CCGGGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
35 CCCTGCGCGGG
BsoBI 17 CCCGGG C^[CT]CG[GA]G
131 CTCGAG
Bsp1286I 178 GAGCTC G[AGT]GC[ACT]^C
341 GTGCAC
BstNI 288 CCTGG CC^[AT]GG
372 CCAGG
Cfr13I 285 GGTCC G^G[ACGT]CC
511 GGACC
DdeI 359 CTCAG C^T[ACGT]AG
533 CTGAG
EcoRII 288 CCTGG ^CC[AT]GG
372 CCAGG
Fnu4HI 6 GCTGC GC^[ACGT]GC
9 GCAGC
HaeII 3 GGCGCT [GA]GCGC^[CT]
46 AGCGCC
HaeIII 60 GGCC GG^CC
591 GGCC
ItaI 6 GCTGC GC^[ACGT]GC
9 GCAGC
MboII 502 GAAGA GAAGA(8/7)
68 TCTTC
MnlI 283 GAGG CCTC(7/6)
535 GAGG
MvaI 288 CCTGG CC^[AT]GG
372 CCAGG
NciI 17 CCCGG CC^[GC]GG
18 CCGGG
PleI 149 GACTC GAGTC(4/5)
505 GAGTC
Sau96I 285 GGTCC G^G[ACGT]CC
511 GGACC
SmlI 131 CTCGAG C^T[CT][GA]AG
175 CTTGAG
TseI 6 GCTGC G^C[AT]GC
9 GCAGC
Tsp45I 317 GTGAC ^GT[GC]AC
415 GTGAC
Tsp509I 529 AATT ^AATT
600 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BssKI CfoI DpnII HhaI
HinP1I HinfI HphI MaeIII MboI
NdeII Sau3AI ScrFI TspRI