Restriction map for
pF1KB6901
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccI AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NcoI NdeI NgoMIV
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
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AatII 211 GACGTC GACGT^C
AccII 132 CGCG CG^CG
AclI 458 AACGTT AA^CGTT
AcsI 239 AAATTC [GA]^AATT[CT]
AlwI 316 GATCC GGATC(4/5)
ApoI 239 AAATTC [GA]^AATT[CT]
Asp700I 235 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 364 GGACC G^G[AT]CC
BanI 93 GGCGCC G^G[CT][GA]CC
BanII 51 GAGCCC G[GA]GC[CT]^C
BbeI 93 GGCGCC GGCGC^C
BbsI 284 GAAGAC GAAGAC(2/6)
BclI 561 TGATCA T^GATCA
BcnI 54 CCCGG CC^[GC]GG
BfaI 596 CTAG C^TAG
BmyI 51 GAGCCC G[AGT]GC[ACT]^C
BpuAI 284 GAAGAC GAAGAC(2/6)
BsiYI 117 CCCGTTGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 117 CCCGTTGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 214 GTCTC GTCTC(1/5)
BsmBI 213 CGTCTC CGTCTC(1/5)
BsmI 272 GAATGC GAATGC(1/-1)
Bsp1286I 51 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 245 TGTACA T^GTACA
BspMI 438 GCAGGT ACCTGC(4/8)
BsrGI 245 TGTACA T^GTACA
BstNI 376 CCAGG CC^[AT]GG
BstUI 132 CGCG CG^CG
BstYI 315 AGATCC [GA]^GATC[CT]
Cfr13I 364 GGACC G^G[ACGT]CC
DdeI 358 CTCAG C^T[ACGT]AG
DsaI 131 CCGCGG C^C[GA][CT]GG
EcoRII 376 CCAGG ^CC[AT]GG
EcoT22I 347 ATGCAT ATGCA^T
FbaI 561 TGATCA T^GATCA
HaeII 93 GGCGCC [GA]GCGC^[CT]
HapII 55 CCGG C^CGG
HgaI 58 GCGTC GACGC(5/10)
HinfI 27 GACTC G^A[ACGT]TC
HpaII 55 CCGG C^CGG
KasI 93 GGCGCC G^GCGCC
KspI 131 CCGCGG CCGC^GG
MaeI 596 CTAG C^TAG
MflI 315 AGATCC [GA]^GATC[CT]
MseI 406 TTAA T^TAA
MspI 55 CCGG C^CGG
MvaI 376 CCAGG CC^[AT]GG
MvnI 132 CGCG CG^CG
NarI 93 GGCGCC GG^CGCC
NciI 54 CCCGG CC^[GC]GG
NheI 595 GCTAGC G^CTAGC
NsiI 347 ATGCAT ATGCA^T
NspI 345 ACATGC [GA]CATG^[CT]
PleI 27 GACTC GAGTC(4/5)
Psp1406I 458 AACGTT AA^CGTT
PstI 389 CTGCAG CTGCA^G
SacII 131 CCGCGG CCGC^GG
Sau96I 364 GGACC G^G[ACGT]CC
SfcI 389 CTGCAG C^T[GA][CT]AG
SfoI 93 GGCGCC GGC^GCC
SspBI 245 TGTACA T^GTACA
SspI 507 AATATT AAT^ATT
Tru9I 406 TTAA T^TAA
Tsp509I 240 AATT ^AATT
TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 315 AGATCC [GA]^GATC[CT]
XmnI 235 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 246 GTAC GT^AC
326 GTAC
AluI 369 AGCT AG^CT
598 AGCT
BseRI 216 CTCCTC GAGGAG(10/8)
355 CTCCTC
BsmFI 379 GGGAC GGGAC(10/14)
60 GTCCC
BssKI 54 CCCGG ^CC[ACGT]GG
376 CCAGG
EarI 279 GAAGAG CTCTTC(1/4)
478 GAAGAG
FauI 42 CCCGC CCCGC(4/6)
221 CCCGC
Ksp632I 279 GAAGAG CTCTTC(1/4)
478 GAAGAG
NlaIII 346 CATG CATG^
559 CATG
NotI 127 GCGGCCGC GC^GGCCGC
160 GCGGCCGC
RsaI 246 GTAC GT^AC
326 GTAC
ScrFI 54 CCCGG CC^[ACGT]GG
376 CCAGG
TaqI 38 TCGA T^CGA
489 TCGA
TthHB8I 38 TCGA T^CGA
489 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcyI BbvI BsaHI BsaJI
BsiEI BstF5I Cac8I CfoI DpnII
EaeI EagI EclXI Eco52I Fnu4HI
FokI HaeIII HhaI Hin1I HinP1I
HphI ItaI MaeII MboI MboII
MnlI MspA1I MwoI NdeII NlaIV
Sau3AI SfaNI TseI