Restriction map for
pF1KB6811
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 382 GACGCC G[GA]^CG[CT]C
AfaI 120 GTAC GT^AC
AlwI 99 GGATC GGATC(4/5)
AspHI 13 GAGCTC G[AT]GC[AT]^C
AvaI 341 CCCGAG C^[CT]CG[GA]G
BanI 328 GGCACC G^G[CT][GA]CC
BanII 13 GAGCTC G[GA]GC[CT]^C
BcnI 435 CCGGG CC^[GC]GG
BlpI 88 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 88 GCTCAGC GC^T[ACGT]AGC
BsaHI 382 GACGCC G[GA]^CG[CT]C
BsiEI 314 CGGCCG CG[GA][CT]^CG
BsiHKAI 13 GAGCTC G[AT]GC[AT]^C
BsmFI 454 GTCCC GGGAC(10/14)
BsoBI 341 CCCGAG C^[CT]CG[GA]G
BspMI 347 ACCTGC ACCTGC(4/8)
BsrBI 86 CCGCTC CCGCTC(-3/-3)
BsrFI 312 GCCGGC [GA]^CCGG[CT]
BssSI 16 CTCGTG CACGAG(-5/-1)
BstAPI 39 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 54 GGTGACC G^GT[ACGT]ACC
BstPI 54 GGTGACC G^GT[ACGT]ACC
CelII 88 GCTCAGC GC^T[ACGT]AGC
Cfr10I 312 GCCGGC [GA]^CCGG[CT]
DdeI 89 CTCAG C^T[ACGT]AG
DpnII 100 GATC ^GATC
DraII 452 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 154 GACAGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 314 CGGCCG C^GGCCG
EclXI 314 CGGCCG C^GGCCG
Eco52I 314 CGGCCG C^GGCCG
EcoO109I 452 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 54 GGTGACC G^GT[ACGT]ACC
FauI 270 GCGGG CCCGC(4/6)
Hin1I 382 GACGCC G[GA]^CG[CT]C
HinfI 124 GAGTC G^A[ACGT]TC
HphI 54 GGTGA GGTGA(8/7)
KspI 62 CCGCGG CCGC^GG
MboI 100 GATC ^GATC
MspA1I 62 CCGCGG C[AC]G^C[GT]G
NaeI 312 GCCGGC GCC^GGC
NciI 435 CCGGG CC^[GC]GG
NdeII 100 GATC ^GATC
NgoMIV 312 GCCGGC G^CCGGC
NlaIII 282 CATG CATG^
NspI 281 GCATGC [GA]CATG^[CT]
PflMI 396 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 124 GAGTC GAGTC(4/5)
PpuMI 452 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 120 GTAC GT^AC
SacI 13 GAGCTC GAGCT^C
SacII 62 CCGCGG CCGC^GG
Sau3AI 100 GATC ^GATC
SfaNI 45 GATGC GCATC(5/9)
SphI 281 GCATGC GCATG^C
Van91I 396 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 33 CGCG CG^CG
63 CGCG
BalI 176 TGGCCA TGG^CCA
413 TGGCCA
BbvI 285 GCAGC GCAGC(8/12)
297 GCAGC
BmyI 13 GAGCTC G[AGT]GC[ACT]^C
327 GGGCAC
BsaI 319 GAGACC GGTCTC(1/5)
344 GAGACC
Bsp1286I 13 GAGCTC G[AGT]GC[ACT]^C
327 GGGCAC
BsrI 234 CCAGT ACTGG(1/-1)
447 CCAGT
BstF5I 231 CATCC GGATG(2/0)
198 GGATG
BstUI 33 CGCG CG^CG
63 CGCG
DsaI 62 CCGCGG C^C[GA][CT]GG
396 CCACGG
FokI 231 CATCC GGATG(9/13)
198 GGATG
HaeII 92 AGCGCC [GA]GCGC^[CT]
287 AGCGCC
HapII 313 CCGG C^CGG
435 CCGG
HgaI 3 GACGC GACGC(5/10)
382 GACGC
HpaII 313 CCGG C^CGG
435 CCGG
MluNI 176 TGGCCA TGG^CCA
413 TGGCCA
MscI 176 TGGCCA TGG^CCA
413 TGGCCA
MspI 313 CCGG C^CGG
435 CCGG
MvnI 33 CGCG CG^CG
63 CGCG
SapI 10 GAAGAGC GCTCTTC(1/4)
390 GAAGAGC
TseI 285 GCAGC G^C[AT]GC
297 GCAGC
TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^
448 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwNI AvaII BsaJI
BseRI BsiYI BslI BsmAI BssKI
BstNI Cac8I CfoI Cfr13I EaeI
EarI EcoRII Fnu4HI HaeIII HhaI
HinP1I ItaI Ksp632I MaeIII MboII
MnlI MvaI MwoI NlaIV Sau96I
ScrFI Tsp45I