Restriction map for
pF1KB8277
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BspDI
BspEI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV EcoT14I EcoT22I
FseI FspI HapII Hin1I HpaI
HpaII KasI KpnI KspI MaeII
MfeI MluI MroI MslI MspA1I
MspI MunI NaeI NarI NciI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 902 CGCG CG^CG
AcsI 970 GAATTC [GA]^AATT[CT]
AlwNI 466 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1041 AGCGCT AGC^GCT
ApoI 970 GAATTC [GA]^AATT[CT]
AvaI 638 CTCGGG C^[CT]CG[GA]G
BalI 330 TGGCCA TGG^CCA
BbsI 363 GAAGAC GAAGAC(2/6)
BfaI 13 CTAG C^TAG
BmyI 718 GTGCCC G[AGT]GC[ACT]^C
BpuAI 363 GAAGAC GAAGAC(2/6)
BsaJI 572 CCCTGG C^C[ACGT][ACGT]GG
BsmI 839 GCATTC GAATGC(1/-1)
BsoBI 638 CTCGGG C^[CT]CG[GA]G
Bsp1286I 718 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 503 TGTACA T^GTACA
BspHI 701 TCATGA T^CATGA
BspMI 369 ACCTGC ACCTGC(4/8)
BsrDI 245 GCAATG GCAATG(2/0)
BsrGI 503 TGTACA T^GTACA
BstEII 213 GGTCACC G^GT[ACGT]ACC
BstPI 213 GGTCACC G^GT[ACGT]ACC
BstUI 902 CGCG CG^CG
BstXI 197 CCAAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 642 GGATCT [GA]^GATC[CT]
CfoI 1042 GCGC GCG^C
DraII 911 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 330 TGGCCA [CT]^GGCC[GA]
Eco47III 1041 AGCGCT AGC^GCT
EcoNI 573 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 911 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 213 GGTCACC G^GT[ACGT]ACC
EcoRI 970 GAATTC G^AATTC
HaeII 1041 AGCGCT [GA]GCGC^[CT]
HhaI 1042 GCGC GCG^C
HinP1I 1042 GCGC G^CGC
HincII 223 GTCAAC GT[CT]^[GA]AC
HindII 223 GTCAAC GT[CT]^[GA]AC
HindIII 398 AAGCTT A^AGCTT
MaeI 13 CTAG C^TAG
MflI 642 GGATCT [GA]^GATC[CT]
MluNI 330 TGGCCA TGG^CCA
MscI 330 TGGCCA TGG^CCA
MvnI 902 CGCG CG^CG
NdeI 122 CATATG CA^TATG
NheI 12 GCTAGC G^CTAGC
NlaIV 418 GGAACC GG[ACGT]^[ACGT]CC
PpuMI 911 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 472 CTGCAG CTGCA^G
RcaI 701 TCATGA T^CATGA
SapI 587 GAAGAGC GCTCTTC(1/4)
SexAI 827 ACCTGGT A^CC[AT]GGT
SfcI 472 CTGCAG C^T[GA][CT]AG
SspBI 503 TGTACA T^GTACA
TfiI 923 GAATC G^A[AT]TC
XcmI 404 CCATGAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 642 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 57 GTCTAC GT^[AC][GT]AC
490 GTCTAC
AflIII 770 ACATGT A^C[GA][CT]GT
950 ACATGT
AlwI 642 GGATC GGATC(4/5)
692 GGATC
AvaII 287 GGACC G^G[AT]CC
912 GGTCC
BclI 99 TGATCA T^GATCA
917 TGATCA
BlpI 84 GCTCAGC GC^T[ACGT]AGC
157 GCTGAGC
Bpu1102I 84 GCTCAGC GC^T[ACGT]AGC
157 GCTGAGC
BsaBI 693 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
918 GATCAGAATC
BsaI 803 GAGACC GGTCTC(1/5)
930 GGTCTC
BsmBI 26 GAGACG CGTCTC(1/5)
340 GAGACG
BsmFI 898 GGGAC GGGAC(10/14)
993 GTCCC
BspLU11I 770 ACATGT A^CATGT
950 ACATGT
BsrBI 160 GAGCGG CCGCTC(-3/-3)
709 GAGCGG
CelII 84 GCTCAGC GC^T[ACGT]AGC
157 GCTGAGC
EarI 587 GAAGAG CTCTTC(1/4)
663 GAAGAG
FbaI 99 TGATCA T^GATCA
917 TGATCA
Ksp632I 587 GAAGAG CTCTTC(1/4)
663 GAAGAG
MamI 693 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
918 GATCAGAATC
MseI 482 TTAA T^TAA
797 TTAA
MwoI 80 GCAAGCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
711 GCGGAGCGTGC
NspI 770 ACATGT [GA]CATG^[CT]
950 ACATGT
Tru9I 482 TTAA T^TAA
797 TTAA
Tsp45I 214 GTCAC ^GT[GC]AC
548 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsiYI BslI BsmAI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp509I TspRI