##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MslI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tsp45I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 400 CGCG CG^CG
AccIII 123 TCCGGA T^CCGGA
AocI 111 CCTGAGG CC^T[ACGT]AGG
AvaII 403 GGTCC G^G[AT]CC
BamHI 116 GGATCC G^GATCC
BsaWI 123 TCCGGA [AT]^CCGG[AT]
BseAI 123 TCCGGA T^CCGGA
BsiYI 231 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 231 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 123 TCCGGA T^CCGGA
BstUI 400 CGCG CG^CG
BstYI 116 GGATCC [GA]^GATC[CT]
Bsu36I 111 CCTGAGG CC^T[ACGT]AGG
Cac8I 348 GCATGC GC[ACGT]^[ACGT]GC
DpnII 117 GATC ^GATC
DsaI 242 CCATGG C^C[GA][CT]GG
EarI 456 CTCTTC CTCTTC(1/4)
Eco81I 111 CCTGAGG CC^T[ACGT]AGG
EcoT14I 242 CCATGG C^C[AT][AT]GG
HapII 124 CCGG C^CGG
HinfI 411 GACTC G^A[ACGT]TC
HpaII 124 CCGG C^CGG
HphI 17 TCACC GGTGA(8/7)
Ksp632I 456 CTCTTC CTCTTC(1/4)
MboI 117 GATC ^GATC
MflI 116 GGATCC [GA]^GATC[CT]
MroI 123 TCCGGA T^CCGGA
MseI 47 TTAA T^TAA
MspA1I 172 CAGCTG C[AC]G^C[GT]G
MspI 124 CCGG C^CGG
MvnI 400 CGCG CG^CG
NcoI 242 CCATGG C^CATGG
NdeII 117 GATC ^GATC
NspI 348 GCATGC [GA]CATG^[CT]
PflMI 231 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 411 GACTC GAGTC(4/5)
PstI 175 CTGCAG CTGCA^G
PvuII 172 CAGCTG CAG^CTG
Sau3AI 117 GATC ^GATC
ScaI 233 AGTACT AGT^ACT
SmlI 376 CTCAAG C^T[CT][GA]AG
SphI 348 GCATGC GCATG^C
StyI 242 CCATGG C^C[AT][AT]GG
Tru9I 47 TTAA T^TAA
Van91I 231 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 116 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 187 GCGG CCGC(-3/-1)
401 GCGG
AfaI 218 GTAC GT^AC
234 GTAC
AlwI 117 GATCC GGATC(4/5)
116 GGATC
BpmI 56 CTCCAG CTGGAG(16/14)
310 CTGGAG
BseRI 82 CTCCTC GAGGAG(10/8)
313 GAGGAG
BsmAI 223 GAGAC GTCTC(1/5)
454 GTCTC
BsmFI 142 GGGAC GGGAC(10/14)
149 GGGAC
BssKI 58 CCAGG ^CC[ACGT]GG
300 CCTGG
BstF5I 126 GGATG GGATG(2/0)
138 GGATG
BstNI 58 CCAGG CC^[AT]GG
300 CCTGG
Cfr13I 102 GGGCC G^G[ACGT]CC
403 GGTCC
DdeI 112 CTGAG C^T[ACGT]AG
181 CTCAG
EcoRII 58 CCAGG ^CC[AT]GG
300 CCTGG
FokI 126 GGATG GGATG(9/13)
138 GGATG
MvaI 58 CCAGG CC^[AT]GG
300 CCTGG
NlaIII 243 CATG CATG^
349 CATG
NlaIV 101 GGGGCC GG[ACGT]^[ACGT]CC
116 GGATCC
RsaI 218 GTAC GT^AC
234 GTAC
Sau96I 102 GGGCC G^G[ACGT]CC
403 GGTCC
ScrFI 58 CCAGG CC^[ACGT]GG
300 CCTGG
SfcI 90 CTGTAG C^T[GA][CT]AG
175 CTGCAG
Tsp509I 64 AATT ^AATT
361 AATT
TspRI 134 CAGTG CA[GC]TG[ACGT][ACGT]^
183 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BalI BbvI BsaJI BsrI
EaeI Fnu4HI HaeIII ItaI MboII
MluNI MnlI MscI MwoI TseI