################################## # NO CUT ENZYMES ( 226 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MflI MluI MluNI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 19 enzymes) # ################################### AccI 280 GTCTAC GT^[AC][GT]AC AciI 67 GCGG CCGC(-3/-1) AseI 52 ATTAAT AT^TAAT AsnI 52 ATTAAT AT^TAAT BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG BsiYI 16 CCCAAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 16 CCCAAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 216 ACCTGC ACCTGC(4/8) EaeI 2 TGGCCG [CT]^GGCC[GA] EcoT14I 17 CCAAGG C^C[AT][AT]GG FauI 67 GCGGG CCCGC(4/6) HaeIII 3 GGCC GG^CC MboII 241 GAAGA GAAGA(8/7) MfeI 248 CAATTG C^AATTG MunI 248 CAATTG C^AATTG PleI 26 GAGTC GAGTC(4/5) PshBI 52 ATTAAT AT^TAAT StyI 17 CCAAGG C^C[AT][AT]GG TfiI 176 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BsgI 218 CTGCAC GTGCAG(16/14) 88 GTGCAG BstF5I 75 GGATG GGATG(2/0) 237 GGATG DdeI 35 CTGAG C^T[ACGT]AG 118 CTTAG DpnII 46 GATC ^GATC 171 GATC FokI 75 GGATG GGATG(9/13) 237 GGATG HinfI 26 GAGTC G^A[ACGT]TC 176 GATTC MboI 46 GATC ^GATC 171 GATC NdeII 46 GATC ^GATC 171 GATC Sau3AI 46 GATC ^GATC 171 GATC Tsp509I 55 AATT ^AATT 249 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### MnlI MseI Tru9I