##################################
# NO CUT ENZYMES (  219 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AciI           23  CCGC                 CCGC(-3/-1)
AvaII           3  GGACC                G^G[AT]CC
BglI          113  GCCCTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          134  GTGCCC               G[AGT]GC[ACT]^C
BsmAI          34  GTCTC                GTCTC(1/5)
Bsp1286I      134  GTGCCC               G[AGT]GC[ACT]^C
BssKI         138  CCAGG                ^CC[ACGT]GG
BstNI         138  CCAGG                CC^[AT]GG
Cac8I          49  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr13I          3  GGACC                G^G[ACGT]CC
EarI           93  GAAGAG               CTCTTC(1/4)
EcoRII        138  CCAGG                ^CC[AT]GG
Ksp632I        93  GAAGAG               CTCTTC(1/4)
MboII          93  GAAGA                GAAGA(8/7)
MnlI           83  CCTC                 CCTC(7/6)
MvaI          138  CCAGG                CC^[AT]GG
NlaIV           3  GGACCC               GG[ACGT]^[ACGT]CC
PstI           26  CTGCAG               CTGCA^G
PvuII         174  CAGCTG               CAG^CTG
SapI           93  GAAGAGC              GCTCTTC(1/4)
Sau96I          3  GGACC                G^G[ACGT]CC
ScrFI         138  CCAGG                CC^[ACGT]GG
SfcI           26  CTGCAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwNI          51  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              174  CAGCTGCTG           
BsaJI         137  CCCAGG               C^C[ACGT][ACGT]GG
              138  CCAGGG              
BsgI           39  CTGCAC               GTGCAG(16/14)
              171  GTGCAG              
BspMI          43  ACCTGC               ACCTGC(4/8)
               28  GCAGGT              
CfoI           47  GCGC                 GCG^C
              182  GCGC                
HhaI           47  GCGC                 GCG^C
              182  GCGC                
HinP1I         47  GCGC                 G^CGC
              182  GCGC                
MspA1I         23  CCGCTG               C[AC]G^C[GT]G
              174  CAGCTG              
SfaNI         146  GCATC                GCATC(5/9)
              160  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AluI        BbvI        BstAPI      Fnu4HI      ItaI        
MwoI        TseI