Restriction map for
pF1KB6575
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StyI SwaI TfiI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AccI 508 GTAGAC GT^[AC][GT]AC
AccII 4 CGCG CG^CG
AcsI 365 AAATTT [GA]^AATT[CT]
Alw44I 374 GTGCAC G^TGCAC
AocI 1027 CCTCAGG CC^T[ACGT]AGG
ApaLI 374 GTGCAC G^TGCAC
ApoI 365 AAATTT [GA]^AATT[CT]
Asp700I 9 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 951 GGACC G^G[AT]CC
BbsI 9 GAAGAC GAAGAC(2/6)
BbvCI 103 GCTGAGG CCTCAGC(-5/-2)
BclI 806 TGATCA T^GATCA
BcnI 1094 CCCGG CC^[GC]GG
BlpI 135 GCTTAGC GC^T[ACGT]AGC
BpmI 59 CTCCAG CTGGAG(16/14)
Bpu1102I 135 GCTTAGC GC^T[ACGT]AGC
BpuAI 9 GAAGAC GAAGAC(2/6)
BsaBI 871 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1109 GAGACC GGTCTC(1/5)
BseRI 446 GAGGAG GAGGAG(10/8)
BsgI 424 GTGCAG GTGCAG(16/14)
BspHI 747 TCATGA T^CATGA
BspMI 454 GCAGGT ACCTGC(4/8)
BsrDI 1067 CATTGC GCAATG(2/0)
BssSI 1106 CACGAG CACGAG(-5/-1)
BstF5I 723 GGATG GGATG(2/0)
BstUI 4 CGCG CG^CG
Bsu36I 1027 CCTCAGG CC^T[ACGT]AGG
Cac8I 523 GCCTGC GC[ACGT]^[ACGT]GC
CelII 135 GCTTAGC GC^T[ACGT]AGC
CfoI 3 GCGC GCG^C
Cfr13I 951 GGACC G^G[ACGT]CC
DpnII 807 GATC ^GATC
DraIII 377 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1027 CCTCAGG CC^T[ACGT]AGG
EcoNI 895 CCTGCATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 153 GATATC GAT^ATC
FbaI 806 TGATCA T^GATCA
FokI 723 GGATG GGATG(9/13)
HapII 1095 CCGG C^CGG
HhaI 3 GCGC GCG^C
HinP1I 3 GCGC G^CGC
HindIII 581 AAGCTT A^AGCTT
HinfI 238 GAGTC G^A[ACGT]TC
HpaII 1095 CCGG C^CGG
MaeII 13 ACGT A^CGT
MamI 871 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 807 GATC ^GATC
MslI 395 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1095 CCGG C^CGG
MvnI 4 CGCG CG^CG
NciI 1094 CCCGG CC^[GC]GG
NdeII 807 GATC ^GATC
PflMI 379 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 238 GAGTC GAGTC(4/5)
RcaI 747 TCATGA T^CATGA
Sau3AI 807 GATC ^GATC
Sau96I 951 GGACC G^G[ACGT]CC
ScaI 1008 AGTACT AGT^ACT
SexAI 378 ACCAGGT A^CC[AT]GGT
SpeI 711 ACTAGT A^CTAGT
SspI 607 AATATT AAT^ATT
StuI 881 AGGCCT AGG^CCT
TaqI 708 TCGA T^CGA
TspRI 260 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 708 TCGA T^CGA
Van91I 379 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 9 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 374 GTGCAC G[AT]GC[AT]^C
1089 GAGCAC
BbvI 30 GCAGC GCAGC(8/12)
922 GCTGC
BsaJI 287 CCCAGG C^C[ACGT][ACGT]GG
288 CCAGGG
BsiHKAI 374 GTGCAC G[AT]GC[AT]^C
1089 GAGCAC
BsmFI 915 GGGAC GGGAC(10/14)
950 GGGAC
BsmI 1000 GAATGC GAATGC(1/-1)
527 GCATTC
Bsp1407I 253 TGTACA T^GTACA
977 TGTACA
BsrGI 253 TGTACA T^GTACA
977 TGTACA
BsrI 261 ACTGG ACTGG(1/-1)
657 ACTGG
BstNI 288 CCAGG CC^[AT]GG
379 CCAGG
EarI 767 CTCTTC CTCTTC(1/4)
194 GAAGAG
EcoRII 288 CCAGG ^CC[AT]GG
379 CCAGG
Fnu4HI 30 GCAGC GC^[ACGT]GC
922 GCTGC
HaeIII 790 GGCC GG^CC
882 GGCC
ItaI 30 GCAGC GC^[ACGT]GC
922 GCTGC
Ksp632I 767 CTCTTC CTCTTC(1/4)
194 GAAGAG
MvaI 288 CCAGG CC^[AT]GG
379 CCAGG
NspI 918 ACATGC [GA]CATG^[CT]
1060 GCATGT
SmlI 271 CTTGAG C^T[CT][GA]AG
1086 CTTGAG
SspBI 253 TGTACA T^GTACA
977 TGTACA
TseI 30 GCAGC G^C[AT]GC
922 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BfaI BmyI
BsiYI BslI BsmAI Bsp1286I BssKI
DdeI FauI HphI MaeI MaeIII
MboII MnlI MseI MspA1I MwoI
NlaIII NlaIV RsaI ScrFI Tru9I
Tsp45I Tsp509I