Restriction map for
pF1KB6422
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspHI AspI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1286I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KspI MaeII MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 490 GGTACC G^GTACC
AccI 661 GTAGAC GT^[AC][GT]AC
AseI 985 ATTAAT AT^TAAT
AsnI 985 ATTAAT AT^TAAT
Asp718I 490 GGTACC G^GTACC
BalI 734 TGGCCA TGG^CCA
BanI 490 GGTACC G^G[CT][GA]CC
BbvCI 226 GCTGAGG CCTCAGC(-5/-2)
BcnI 447 CCGGG CC^[GC]GG
BglI 501 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 404 AGATCT A^GATCT
BsaI 535 GGTCTC GGTCTC(1/5)
BsaJI 447 CCGGGG C^C[ACGT][ACGT]GG
Bsp1407I 473 TGTACA T^GTACA
BspMI 427 ACCTGC ACCTGC(4/8)
BsrDI 4 GCAATG GCAATG(2/0)
BsrGI 473 TGTACA T^GTACA
CfoI 163 GCGC GCG^C
Cfr13I 61 GGCCC G^G[ACGT]CC
EaeI 734 TGGCCA [CT]^GGCC[GA]
EarI 914 GAAGAG CTCTTC(1/4)
EcoNI 856 CCTATTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 881 ATGCAT ATGCA^T
HapII 447 CCGG C^CGG
HhaI 163 GCGC GCG^C
HinP1I 163 GCGC G^CGC
HindIII 54 AAGCTT A^AGCTT
HpaII 447 CCGG C^CGG
KpnI 490 GGTACC GGTAC^C
Ksp632I 914 GAAGAG CTCTTC(1/4)
MaeIII 424 GTCAC ^GT[ACGT]AC
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
MslI 366 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 447 CCGG C^CGG
NciI 447 CCGGG CC^[GC]GG
NdeI 569 CATATG CA^TATG
NheI 417 GCTAGC G^CTAGC
NsiI 881 ATGCAT ATGCA^T
PaeR7I 575 CTCGAG C^TCGAG
PflMI 138 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 985 ATTAAT AT^TAAT
Sau96I 61 GGCCC G^G[ACGT]CC
SspBI 473 TGTACA T^GTACA
SspI 184 AATATT AAT^ATT
Tsp45I 424 GTCAC ^GT[GC]AC
Van91I 138 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 575 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 474 GTAC GT^AC
491 GTAC
AlwI 844 GATCC GGATC(4/5)
358 GGATC
AlwNI 518 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
927 CAGAATCTG
AvaI 575 CTCGAG C^[CT]CG[GA]G
696 CTCGGG
BbsI 396 GAAGAC GAAGAC(2/6)
956 GTCTTC
BfaI 418 CTAG C^TAG
641 CTAG
BpuAI 396 GAAGAC GAAGAC(2/6)
956 GTCTTC
BsmAI 352 GAGAC GTCTC(1/5)
536 GTCTC
BsoBI 575 CTCGAG C^[CT]CG[GA]G
696 CTCGGG
BstF5I 749 GGATG GGATG(2/0)
819 GGATG
BstYI 358 GGATCT [GA]^GATC[CT]
404 AGATCT
EcoRI 271 GAATTC G^AATTC
382 GAATTC
FokI 749 GGATG GGATG(9/13)
819 GGATG
MaeI 418 CTAG C^TAG
641 CTAG
MflI 358 GGATCT [GA]^GATC[CT]
404 AGATCT
MseI 188 TTAA T^TAA
986 TTAA
NlaIV 313 GGCTCC GG[ACGT]^[ACGT]CC
490 GGTACC
PleI 761 GACTC GAGTC(4/5)
946 GACTC
RsaI 474 GTAC GT^AC
491 GTAC
SmlI 575 CTCGAG C^T[CT][GA]AG
812 CTCAAG
TaqI 576 TCGA T^CGA
702 TCGA
Tru9I 188 TTAA T^TAA
986 TTAA
TthHB8I 576 TCGA T^CGA
702 TCGA
XhoII 358 GGATCT [GA]^GATC[CT]
404 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsiYI BslI BsrI BssKI BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI TfiI
TseI Tsp509I TspRI