Restriction map for
pF1KB6460
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 52 GAATTC [GA]^AATT[CT]
Aor51HI 839 AGCGCT AGC^GCT
ApaI 668 GGGCCC GGGCC^C
ApoI 52 GAATTC [GA]^AATT[CT]
AvaI 319 CTCGAG C^[CT]CG[GA]G
BanI 601 GGCACC G^G[CT][GA]CC
BciVI 969 GGATAC GTATCC(6/5)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaBI 909 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 472 CTGCAC GTGCAG(16/14)
BsmBI 753 CGTCTC CGTCTC(1/5)
BsoBI 319 CTCGAG C^[CT]CG[GA]G
BsrBI 101 CCGCTC CCGCTC(-3/-3)
DraI 955 TTTAAA TTT^AAA
DsaI 656 CCGTGG C^C[GA][CT]GG
EaeI 695 TGGCCG [CT]^GGCC[GA]
Eco47III 839 AGCGCT AGC^GCT
EcoNI 816 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 52 GAATTC G^AATTC
EcoRV 871 GATATC GAT^ATC
HaeII 839 AGCGCT [GA]GCGC^[CT]
MaeII 368 ACGT A^CGT
MamI 909 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 698 CCGCTG C[AC]G^C[GT]G
PaeR7I 319 CTCGAG C^TCGAG
PstI 820 CTGCAG CTGCA^G
SbfI 819 CCTGCAGG CCTGCA^GG
Sse8387I 819 CCTGCAGG CCTGCA^GG
Tsp45I 547 GTGAC ^GT[GC]AC
Tsp509I 53 AATT ^AATT
TspRI 417 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 319 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 441 CGCG CG^CG
571 CGCG
AfaI 366 GTAC GT^AC
612 GTAC
Asp700I 48 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
73 GAAGATTTTC
BbvI 29 GCAGC GCAGC(8/12)
807 GCTGC
BglII 737 AGATCT A^GATCT
828 AGATCT
BsaI 193 GAGACC GGTCTC(1/5)
1011 GGTCTC
BsmFI 497 GGGAC GGGAC(10/14)
98 GTCCC
BspMI 803 ACCTGC ACCTGC(4/8)
822 GCAGGT
BsrI 662 ACTGG ACTGG(1/-1)
905 ACTGG
BstF5I 282 CATCC GGATG(2/0)
732 CATCC
BstUI 441 CGCG CG^CG
571 CGCG
FokI 282 CATCC GGATG(9/13)
732 CATCC
HincII 295 GTCAAC GT[CT]^[GA]AC
865 GTCAAC
HindII 295 GTCAAC GT[CT]^[GA]AC
865 GTCAAC
HinfI 137 GAATC G^A[ACGT]TC
513 GAATC
MaeIII 547 GTGAC ^GT[ACGT]AC
977 GTAAC
MseI 83 TTAA T^TAA
956 TTAA
MslI 354 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
540 CATTCAGGTG
MvnI 441 CGCG CG^CG
571 CGCG
PpuMI 497 GGGACCT [GA]G^G[AT]CC[CT]
800 AGGACCT
RsaI 366 GTAC GT^AC
612 GTAC
SfaNI 174 GATGC GCATC(5/9)
281 GCATC
SfcI 492 CTACAG C^T[GA][CT]AG
820 CTGCAG
SmlI 319 CTCGAG C^T[CT][GA]AG
853 CTCAAG
TfiI 137 GAATC G^A[AT]TC
513 GAATC
Tru9I 83 TTAA T^TAA
956 TTAA
TseI 29 GCAGC G^C[AT]GC
807 GCTGC
XmnI 48 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
73 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TaqI TthHB8I XhoII