Restriction map for
pF1KE0102
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrGI Bst1107I BstAPI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 504 CGCG CG^CG
AlwNI 1045 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 50 CCTCAGG CC^T[ACGT]AGG
ApaI 657 GGGCCC GGGCC^C
Asp700I 444 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 137 TGGCCA TGG^CCA
BamHI 926 GGATCC G^GATCC
BlpI 571 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC
BseRI 195 CTCCTC GAGGAG(10/8)
BsmAI 120 GTCTC GTCTC(1/5)
BsrBI 372 GAGCGG CCGCTC(-3/-3)
BsrDI 233 GCAATG GCAATG(2/0)
BsrFI 1061 ACCGGC [GA]^CCGG[CT]
BssHII 503 GCGCGC G^CGCGC
BssSI 349 CACGAG CACGAG(-5/-1)
BstBI 608 TTCGAA TT^CGAA
BstEII 69 GGTTACC G^GT[ACGT]ACC
BstF5I 324 GGATG GGATG(2/0)
BstPI 69 GGTTACC G^GT[ACGT]ACC
BstUI 504 CGCG CG^CG
BstYI 926 GGATCC [GA]^GATC[CT]
Bsu36I 50 CCTCAGG CC^T[ACGT]AGG
CelII 571 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1061 ACCGGC [GA]^CCGG[CT]
EaeI 137 TGGCCA [CT]^GGCC[GA]
Eco81I 50 CCTCAGG CC^T[ACGT]AGG
EcoO65I 69 GGTTACC G^GT[ACGT]ACC
FokI 324 GGATG GGATG(9/13)
HaeII 635 GGCGCT [GA]GCGC^[CT]
HindIII 1035 AAGCTT A^AGCTT
MflI 926 GGATCC [GA]^GATC[CT]
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MslI 387 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 504 CGCG CG^CG
NspV 608 TTCGAA TT^CGAA
SapI 720 GAAGAGC GCTCTTC(1/4)
SexAI 286 ACCTGGT A^CC[AT]GGT
SfuI 608 TTCGAA TT^CGAA
StuI 446 AGGCCT AGG^CCT
Tsp45I 526 GTGAC ^GT[GC]AC
Tsp509I 188 AATT ^AATT
XhoII 926 GGATCC [GA]^GATC[CT]
XmnI 444 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 464 GAGCCC G[GA]GC[CT]^C
657 GGGCCC
BclI 533 TGATCA T^GATCA
1010 TGATCA
BsmFI 796 GGGAC GGGAC(10/14)
951 GGGAC
BspHI 83 TCATGA T^CATGA
1007 TCATGA
BsrI 313 ACTGG ACTGG(1/-1)
698 ACTGG
EarI 79 CTCTTC CTCTTC(1/4)
720 GAAGAG
FbaI 533 TGATCA T^GATCA
1010 TGATCA
Ksp632I 79 CTCTTC CTCTTC(1/4)
720 GAAGAG
MaeII 98 ACGT A^CGT
830 ACGT
MaeIII 70 GTTAC ^GT[ACGT]AC
526 GTGAC
MspA1I 124 CAGCGG C[AC]G^C[GT]G
276 CCGCTG
NspI 576 GCATGT [GA]CATG^[CT]
894 GCATGT
PflMI 36 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
161 CCAGACTGTGG
PpuMI 11 AGGACCC [GA]G^G[AT]CC[CT]
361 AGGACCC
RcaI 83 TCATGA T^CATGA
1007 TCATGA
Van91I 36 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
161 CCAGACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TspRI TthHB8I