Restriction map for
pF1KB6413
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MseI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 571 GACGTC GACGT^C
AccI 868 GTCGAC GT^[AC][GT]AC
AccIII 35 TCCGGA T^CCGGA
AcyI 571 GACGTC G[GA]^CG[CT]C
AfaI 153 GTAC GT^AC
AflIII 329 ACGTGT A^C[GA][CT]GT
AlwNI 808 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 805 CCTCAGG CC^T[ACGT]AGG
ApaI 581 GGGCCC GGGCC^C
AvaI 421 CTCGAG C^[CT]CG[GA]G
AvaII 810 GGTCC G^G[AT]CC
BalI 50 TGGCCA TGG^CCA
BfaI 169 CTAG C^TAG
BpmI 127 CTGGAG CTGGAG(16/14)
BsaHI 571 GACGTC G[GA]^CG[CT]C
BsaWI 35 TCCGGA [AT]^CCGG[AT]
BseAI 35 TCCGGA T^CCGGA
BsiEI 200 CGGCCG CG[GA][CT]^CG
BsmBI 118 GAGACG CGTCTC(1/5)
BsoBI 421 CTCGAG C^[CT]CG[GA]G
BspEI 35 TCCGGA T^CCGGA
BsrFI 77 GCCGGC [GA]^CCGG[CT]
BstXI 689 CCACCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 344 GGATCT [GA]^GATC[CT]
Bsu36I 805 CCTCAGG CC^T[ACGT]AGG
Cfr10I 77 GCCGGC [GA]^CCGG[CT]
EagI 200 CGGCCG C^GGCCG
EclXI 200 CGGCCG C^GGCCG
Eco52I 200 CGGCCG C^GGCCG
Eco81I 805 CCTCAGG CC^T[ACGT]AGG
EcoT22I 841 ATGCAT ATGCA^T
Hin1I 571 GACGTC G[GA]^CG[CT]C
HincII 868 GTCGAC GT[CT]^[GA]AC
HindII 868 GTCGAC GT[CT]^[GA]AC
HinfI 264 GACTC G^A[ACGT]TC
KspI 709 CCGCGG CCGC^GG
MaeI 169 CTAG C^TAG
MflI 344 GGATCT [GA]^GATC[CT]
MluNI 50 TGGCCA TGG^CCA
MroI 35 TCCGGA T^CCGGA
MscI 50 TGGCCA TGG^CCA
MslI 690 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 77 GCCGGC GCC^GGC
NgoMIV 77 GCCGGC G^CCGGC
NsiI 841 ATGCAT ATGCA^T
NspI 839 ACATGC [GA]CATG^[CT]
PaeR7I 421 CTCGAG C^TCGAG
PleI 264 GACTC GAGTC(4/5)
PpuMI 809 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 253 CTGCAG CTGCA^G
RsaI 153 GTAC GT^AC
SacII 709 CCGCGG CCGC^GG
SalI 868 GTCGAC G^TCGAC
SapI 609 GAAGAGC GCTCTTC(1/4)
StuI 488 AGGCCT AGG^CCT
Tsp45I 262 GTGAC ^GT[GC]AC
Tsp509I 288 AATT ^AATT
XcmI 446 CCAAGCGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 421 CTCGAG C^TCGAG
XhoII 344 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanI 80 GGCACC G^G[CT][GA]CC
705 GGTGCC
BanII 39 GAGCCC G[GA]GC[CT]^C
581 GGGCCC
BmyI 39 GAGCCC G[AGT]GC[ACT]^C
581 GGGCCC
BseRI 190 GAGGAG GAGGAG(10/8)
646 GAGGAG
BsgI 441 CTGCAC GTGCAG(16/14)
594 CTGCAC
Bsp1286I 39 GAGCCC G[AGT]GC[ACT]^C
581 GGGCCC
BspMI 632 ACCTGC ACCTGC(4/8)
516 GCAGGT
EcoT14I 182 CCAAGG C^C[AT][AT]GG
524 CCAAGG
HaeII 27 GGCGCT [GA]GCGC^[CT]
679 AGCGCC
HapII 36 CCGG C^CGG
78 CCGG
HpaII 36 CCGG C^CGG
78 CCGG
MaeII 329 ACGT A^CGT
572 ACGT
MaeIII 262 GTGAC ^GT[ACGT]AC
469 GTTAC
MspI 36 CCGG C^CGG
78 CCGG
SfaNI 135 GATGC GCATC(5/9)
369 GATGC
SfcI 227 CTACAG C^T[GA][CT]AG
253 CTGCAG
StyI 182 CCAAGG C^C[AT][AT]GG
524 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HphI ItaI
Ksp632I MboI MboII MnlI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SmlI
TaqI TseI TspRI TthHB8I