Restriction map for
pF1KE0876
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoT22I FauI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwI 542 GGATC GGATC(4/5)
AocI 969 CCTCAGG CC^T[ACGT]AGG
AseI 778 ATTAAT AT^TAAT
AsnI 778 ATTAAT AT^TAAT
AvaII 536 GGTCC G^G[AT]CC
BalI 467 TGGCCA TGG^CCA
BbvCI 787 GCTGAGG CCTCAGC(-5/-2)
BbvI 529 GCTGC GCAGC(8/12)
BclI 618 TGATCA T^GATCA
BfaI 88 CTAG C^TAG
BpmI 163 CTCCAG CTGGAG(16/14)
BseRI 226 CTCCTC GAGGAG(10/8)
BsmAI 100 GAGAC GTCTC(1/5)
BsmFI 405 GTCCC GGGAC(10/14)
BspMI 79 GCAGGT ACCTGC(4/8)
BsrI 714 ACTGG ACTGG(1/-1)
BstEII 82 GGTGACC G^GT[ACGT]ACC
BstPI 82 GGTGACC G^GT[ACGT]ACC
Bsu36I 969 CCTCAGG CC^T[ACGT]AGG
Cac8I 75 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 823 GCGC GCG^C
EaeI 467 TGGCCA [CT]^GGCC[GA]
EarI 762 CTCTTC CTCTTC(1/4)
Eco81I 969 CCTCAGG CC^T[ACGT]AGG
EcoO65I 82 GGTGACC G^GT[ACGT]ACC
EcoRI 127 GAATTC G^AATTC
EcoRV 667 GATATC GAT^ATC
EcoT14I 408 CCTTGG C^C[AT][AT]GG
FbaI 618 TGATCA T^GATCA
Fnu4HI 529 GCTGC GC^[ACGT]GC
HhaI 823 GCGC GCG^C
HinP1I 823 GCGC G^CGC
ItaI 529 GCTGC GC^[ACGT]GC
Ksp632I 762 CTCTTC CTCTTC(1/4)
MaeI 88 CTAG C^TAG
MluNI 467 TGGCCA TGG^CCA
MscI 467 TGGCCA TGG^CCA
NlaIV 418 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 459 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 778 ATTAAT AT^TAAT
PstI 391 CTGCAG CTGCA^G
SacI 160 GAGCTC GAGCT^C
SspI 703 AATATT AAT^ATT
StyI 408 CCTTGG C^C[AT][AT]GG
TaqI 829 TCGA T^CGA
TseI 529 GCTGC G^C[AT]GC
TthHB8I 829 TCGA T^CGA
Van91I 459 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 127 GAATTC [GA]^AATT[CT]
635 AAATTT
AluI 161 AGCT AG^CT
167 AGCT
ApoI 127 GAATTC [GA]^AATT[CT]
635 AAATTT
Asp700I 127 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAAGCCTTC
AspHI 160 GAGCTC G[AT]GC[AT]^C
386 GTGCTC
BanII 160 GAGCTC G[GA]GC[CT]^C
417 GGGCTC
BsaJI 213 CCTGGG C^C[ACGT][ACGT]GG
408 CCTTGG
BsiHKAI 160 GAGCTC G[AT]GC[AT]^C
386 GTGCTC
BspHI 500 TCATGA T^CATGA
689 TCATGA
Cfr13I 536 GGTCC G^G[ACGT]CC
642 GGCCC
DpnII 543 GATC ^GATC
619 GATC
DraIII 423 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
460 CACTATGTG
MboI 543 GATC ^GATC
619 GATC
MwoI 442 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
513 GCTGTATGTGC
NdeII 543 GATC ^GATC
619 GATC
RcaI 500 TCATGA T^CATGA
689 TCATGA
Sau3AI 543 GATC ^GATC
619 GATC
Sau96I 536 GGTCC G^G[ACGT]CC
642 GGCCC
SfcI 391 CTGCAG C^T[GA][CT]AG
963 CTATAG
Tsp45I 83 GTGAC ^GT[GC]AC
697 GTGAC
XmnI 127 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAAGCCTTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI BmyI BsiYI BslI Bsp1286I
Bsp1407I BsrGI BssKI BstF5I BstNI
DdeI EcoRII FokI HaeIII HinfI
HphI MaeIII MboII MnlI MseI
MslI MvaI NlaIII RsaI ScrFI
SspBI TfiI Tru9I Tsp509I TspRI