Restriction map for
pF1KB6373
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HpaI HphI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 790 GACGCC G[GA]^CG[CT]C
AlwI 71 GGATC GGATC(4/5)
AocI 295 CCTGAGG CC^T[ACGT]AGG
AvaI 101 CCCGAG C^[CT]CG[GA]G
AvaII 563 GGACC G^G[AT]CC
AvrII 604 CCTAGG C^CTAGG
BbsI 207 GAAGAC GAAGAC(2/6)
BbvCI 664 GCTGAGG CCTCAGC(-5/-2)
BfaI 605 CTAG C^TAG
BlnI 604 CCTAGG C^CTAGG
BpuAI 207 GAAGAC GAAGAC(2/6)
BsaHI 790 GACGCC G[GA]^CG[CT]C
BsmFI 349 GGGAC GGGAC(10/14)
BsoBI 101 CCCGAG C^[CT]CG[GA]G
BspMI 383 ACCTGC ACCTGC(4/8)
BssHII 78 GCGCGC G^CGCGC
BssSI 730 CTCGTG CACGAG(-5/-1)
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
DpnII 72 GATC ^GATC
DsaI 785 CCGTGG C^C[GA][CT]GG
EarI 390 GAAGAG CTCTTC(1/4)
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
EcoNI 144 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 799 GATATC GAT^ATC
HgaI 790 GACGC GACGC(5/10)
Hin1I 790 GACGCC G[GA]^CG[CT]C
HindIII 497 AAGCTT A^AGCTT
Ksp632I 390 GAAGAG CTCTTC(1/4)
MaeI 605 CTAG C^TAG
MaeII 352 ACGT A^CGT
MboI 72 GATC ^GATC
MseI 592 TTAA T^TAA
MslI 780 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 521 CATATG CA^TATG
NdeII 72 GATC ^GATC
NspI 626 GCATGC [GA]CATG^[CT]
PvuII 325 CAGCTG CAG^CTG
SacI 154 GAGCTC GAGCT^C
Sau3AI 72 GATC ^GATC
SfaNI 59 GCATC GCATC(5/9)
SfiI 646 GGCCGCATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 626 GCATGC GCATG^C
StuI 292 AGGCCT AGG^CCT
TfiI 317 GATTC G^A[AT]TC
Tru9I 592 TTAA T^TAA
XcmI 550 CCAGGAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 354 GTAC GT^AC
756 GTAC
AlwNI 325 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
637 CAGCCACTG
ApaI 98 GGGCCC GGGCC^C
140 GGGCCC
AspHI 154 GAGCTC G[AT]GC[AT]^C
777 GAGCAC
BalI 270 TGGCCA TGG^CCA
464 TGGCCA
BglI 233 GCCGATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
647 GCCGCATGGGC
BsaI 223 GAGACC GGTCTC(1/5)
301 GAGACC
BseRI 298 GAGGAG GAGGAG(10/8)
577 GAGGAG
BsgI 711 CTGCAC GTGCAG(16/14)
743 GTGCAG
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
777 GAGCAC
BsmAI 223 GAGAC GTCTC(1/5)
301 GAGAC
BstXI 419 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
494 CCAAAGCTTTGG
DraII 97 GGGGCCC [GA]G^G[ACGT]CC[CT]
140 GGGCCCT
EcoO109I 97 GGGGCCC [GA]G^G[ACGT]CC[CT]
140 GGGCCCT
EcoT14I 470 CCAAGG C^C[AT][AT]GG
604 CCTAGG
HincII 259 GTCAAC GT[CT]^[GA]AC
535 GTCAAC
HindII 259 GTCAAC GT[CT]^[GA]AC
535 GTCAAC
MluNI 270 TGGCCA TGG^CCA
464 TGGCCA
MscI 270 TGGCCA TGG^CCA
464 TGGCCA
PflMI 324 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCAGCCACTGG
RsaI 354 GTAC GT^AC
756 GTAC
StyI 470 CCAAGG C^C[AT][AT]GG
604 CCTAGG
TspRI 641 CACTG CA[GC]TG[ACGT][ACGT]^
683 CAGTG
Van91I 324 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
Sau96I ScrFI TseI Tsp45I