Restriction map for
pF1KB6565
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaLI ApoI AscI AseI
AsnI AspEI AviII AvrII BbrPI
BbvCI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BsgI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MaeI MaeII
MfeI MluI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 389 GGTACC G^GTACC
AccIII 809 TCCGGA T^CCGGA
Aor51HI 815 AGCGCT AGC^GCT
ApaI 787 GGGCCC GGGCC^C
Asp700I 480 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 389 GGTACC G^GTACC
AspI 1017 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 24 GGTCC G^G[AT]CC
BbeI 73 GGCGCC GGCGC^C
BcgI 971 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 729 AGATCT A^GATCT
BsaBI 522 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 809 TCCGGA T^CCGGA
BseRI 196 GAGGAG GAGGAG(10/8)
BsmAI 1015 GAGAC GTCTC(1/5)
BsmBI 1015 GAGACG CGTCTC(1/5)
BsmFI 422 GGGAC GGGAC(10/14)
BsmI 706 GCATTC GAATGC(1/-1)
BspEI 809 TCCGGA T^CCGGA
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BssHII 55 GCGCGC G^CGCGC
BstEII 588 GGTCACC G^GT[ACGT]ACC
BstF5I 831 GGATG GGATG(2/0)
BstPI 588 GGTCACC G^GT[ACGT]ACC
DdeI 124 CTGAG C^T[ACGT]AG
DraII 786 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco47III 815 AGCGCT AGC^GCT
Eco52I 43 CGGCCG C^GGCCG
EcoO109I 786 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 588 GGTCACC G^GT[ACGT]ACC
FauI 936 CCCGC CCCGC(4/6)
FokI 831 GGATG GGATG(9/13)
KasI 73 GGCGCC G^GCGCC
KpnI 389 GGTACC GGTAC^C
MamI 522 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 809 TCCGGA T^CCGGA
MspA1I 40 CAGCGG C[AC]G^C[GT]G
NaeI 555 GCCGGC GCC^GGC
NarI 73 GGCGCC GG^CGCC
NcoI 827 CCATGG C^CATGG
NgoMIV 555 GCCGGC G^CCGGC
PflFI 1017 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 73 GGCGCC GGC^GCC
TfiI 137 GAATC G^A[AT]TC
Tth111I 1017 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 957 CCACTGGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 480 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 473 GTGCTC G[AT]GC[AT]^C
532 GAGCAC
AvaI 132 CTCGGG C^[CT]CG[GA]G
878 CCCGAG
BalI 2 TGGCCA TGG^CCA
961 TGGCCA
BamHI 81 GGATCC G^GATCC
323 GGATCC
BpmI 747 CTCCAG CTGGAG(16/14)
28 CTGGAG
BsaWI 143 ACCGGA [AT]^CCGG[AT]
809 TCCGGA
BsiEI 43 CGGCCG CG[GA][CT]^CG
990 CGACCG
BsiHKAI 473 GTGCTC G[AT]GC[AT]^C
532 GAGCAC
BsoBI 132 CTCGGG C^[CT]CG[GA]G
878 CCCGAG
BsrFI 281 ACCGGC [GA]^CCGG[CT]
555 GCCGGC
Cfr10I 281 ACCGGC [GA]^CCGG[CT]
555 GCCGGC
HaeII 73 GGCGCC [GA]GCGC^[CT]
815 AGCGCT
MluNI 2 TGGCCA TGG^CCA
961 TGGCCA
MscI 2 TGGCCA TGG^CCA
961 TGGCCA
PleI 256 GAGTC GAGTC(4/5)
1021 GAGTC
SbfI 582 CCTGCAGG CCTGCA^GG
696 CCTGCAGG
SfaNI 499 GATGC GCATC(5/9)
971 GCATC
Sse8387I 582 CCTGCAGG CCTGCA^GG
696 CCTGCAGG
TaqI 312 TCGA T^CGA
530 TCGA
Tsp45I 589 GTCAC ^GT[GC]AC
982 GTCAC
TthHB8I 312 TCGA T^CGA
530 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BanII BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaJI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TseI TspRI
XhoII