Restriction map for
pF1KB6349
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 264 GTAC GT^AC
AlwI 528 GATCC GGATC(4/5)
AocI 376 CCTAAGG CC^T[ACGT]AGG
Aor51HI 39 AGCGCT AGC^GCT
Asp700I 571 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 545 GGACC G^G[AT]CC
BanII 66 GAGCTC G[GA]GC[CT]^C
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BseRI 190 GAGGAG GAGGAG(10/8)
BsmAI 539 GAGAC GTCTC(1/5)
BspMI 601 ACCTGC ACCTGC(4/8)
BsrBI 151 GAGCGG CCGCTC(-3/-3)
Bsu36I 376 CCTAAGG CC^T[ACGT]AGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
DpnII 528 GATC ^GATC
DsaI 305 CCGCGG C^C[GA][CT]GG
EaeI 489 CGGCCA [CT]^GGCC[GA]
Eco47III 39 AGCGCT AGC^GCT
Eco81I 376 CCTAAGG CC^T[ACGT]AGG
EcoNI 288 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 723 CCTTGG C^C[AT][AT]GG
HaeII 39 AGCGCT [GA]GCGC^[CT]
HgaI 256 GACGC GACGC(5/10)
KspI 305 CCGCGG CCGC^GG
MboI 528 GATC ^GATC
NdeII 528 GATC ^GATC
NlaIII 33 CATG CATG^
PflMI 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 264 GTAC GT^AC
SacI 66 GAGCTC GAGCT^C
SacII 305 CCGCGG CCGC^GG
SapI 717 GCTCTTC GCTCTTC(1/4)
Sau3AI 528 GATC ^GATC
ScaI 263 AGTACT AGT^ACT
StuI 522 AGGCCT AGG^CCT
StyI 723 CCTTGG C^C[AT][AT]GG
Van91I 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 100 CCACGTTTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 571 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 299 CGCG CG^CG
306 CGCG
AlwNI 406 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
553 CAGACCCTG
BcnI 228 CCGGG CC^[GC]GG
731 CCGGG
BpmI 20 CTGGAG CTGGAG(16/14)
289 CTGGAG
BsgI 668 CTGCAC GTGCAG(16/14)
358 GTGCAG
BsmFI 89 GGGAC GGGAC(10/14)
145 GGGAC
BstF5I 285 CATCC GGATG(2/0)
509 GGATG
BstUI 299 CGCG CG^CG
306 CGCG
EarI 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
FauI 153 GCGGG CCCGC(4/6)
300 GCGGG
FokI 285 CATCC GGATG(9/13)
509 GGATG
HapII 228 CCGG C^CGG
731 CCGG
HpaII 228 CCGG C^CGG
731 CCGG
Ksp632I 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
MaeII 102 ACGT A^CGT
215 ACGT
MspA1I 305 CCGCGG C[AC]G^C[GT]G
361 CAGCGG
MspI 228 CCGG C^CGG
731 CCGG
MvnI 299 CGCG CG^CG
306 CGCG
NciI 228 CCGGG CC^[GC]GG
731 CCGGG
PstI 321 CTGCAG CTGCA^G
412 CTGCAG
SfaNI 564 GATGC GCATC(5/9)
681 GATGC
SfcI 321 CTGCAG C^T[GA][CT]AG
412 CTGCAG
TaqI 206 TCGA T^CGA
582 TCGA
TthHB8I 206 TCGA T^CGA
582 TCGA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboII MnlI MvaI MwoI
NlaIV PleI Sau96I ScrFI TfiI
TseI Tsp45I TspRI