Restriction map for
pF1KB6342
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BsrI BssSI Bst1107I BstBI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI DraIII Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
Sau3AI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp509I TspRI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AlwNI 634 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 309 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 111 CCCGAG C^[CT]CG[GA]G
BanI 777 GGCGCC G^G[CT][GA]CC
BanII 841 GAGCCC G[GA]GC[CT]^C
BbeI 777 GGCGCC GGCGC^C
BbrPI 748 CACGTG CAC^GTG
BbsI 817 GAAGAC GAAGAC(2/6)
BfaI 560 CTAG C^TAG
BlpI 426 GCTGAGC GC^T[ACGT]AGC
BpmI 715 CTGGAG CTGGAG(16/14)
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaAI 748 CACGTG [CT]AC^GT[GA]
BsmAI 523 GAGAC GTCTC(1/5)
BsmBI 523 GAGACG CGTCTC(1/5)
BsoBI 111 CCCGAG C^[CT]CG[GA]G
BspMI 699 GCAGGT ACCTGC(4/8)
BsrDI 450 CATTGC GCAATG(2/0)
BssHII 148 GCGCGC G^CGCGC
BstAPI 696 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 426 GCTGAGC GC^T[ACGT]AGC
DrdI 525 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 480 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 81 GGCCGGCC GGCCGG^CC
HinfI 250 GACTC G^A[ACGT]TC
KasI 777 GGCGCC G^GCGCC
MaeI 560 CTAG C^TAG
MslI 88 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 82 GCCGGC GCC^GGC
NarI 777 GGCGCC GG^CGCC
NgoMIV 82 GCCGGC G^CCGGC
NspI 737 ACATGC [GA]CATG^[CT]
PflFI 309 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 250 GACTC GAGTC(4/5)
PmaCI 748 CACGTG CAC^GTG
PmlI 748 CACGTG CAC^GTG
PpuMI 504 GGGACCT [GA]G^G[AT]CC[CT]
PstI 493 CTGCAG CTGCA^G
PvuII 142 CAGCTG CAG^CTG
SbfI 492 CCTGCAGG CCTGCA^GG
SexAI 260 ACCAGGT A^CC[AT]GGT
SfaNI 204 GATGC GCATC(5/9)
SfoI 777 GGCGCC GGC^GCC
Sse8387I 492 CCTGCAGG CCTGCA^GG
StuI 791 AGGCCT AGG^CCT
Tth111I 309 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 312 GGCGTC G[GA]^CG[CT]C
777 GGCGCC
Asp700I 120 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
438 GAACGCCTTC
AspHI 177 GAGCAC G[AT]GC[AT]^C
865 GAGCAC
BbvCI 454 GCTGAGG CCTCAGC(-5/-2)
799 GCTGAGG
BsaHI 312 GGCGTC G[GA]^CG[CT]C
777 GGCGCC
BsiEI 80 CGGCCG CG[GA][CT]^CG
590 CGGCCG
BsiHKAI 177 GAGCAC G[AT]GC[AT]^C
865 GAGCAC
BsmFI 504 GGGAC GGGAC(10/14)
579 GGGAC
BsrFI 82 GCCGGC [GA]^CCGG[CT]
330 GCCGGT
BstEII 333 GGTGACC G^GT[ACGT]ACC
657 GGTGACC
BstF5I 93 GGATG GGATG(2/0)
519 GGATG
BstPI 333 GGTGACC G^GT[ACGT]ACC
657 GGTGACC
Cfr10I 82 GCCGGC [GA]^CCGG[CT]
330 GCCGGT
DraII 488 AGGGCCT [GA]G^G[ACGT]CC[CT]
504 GGGACCT
EagI 80 CGGCCG C^GGCCG
590 CGGCCG
EclXI 80 CGGCCG C^GGCCG
590 CGGCCG
Eco52I 80 CGGCCG C^GGCCG
590 CGGCCG
EcoO109I 488 AGGGCCT [GA]G^G[ACGT]CC[CT]
504 GGGACCT
EcoO65I 333 GGTGACC G^GT[ACGT]ACC
657 GGTGACC
FokI 93 GGATG GGATG(9/13)
519 GGATG
HgaI 525 GACGC GACGC(5/10)
313 GCGTC
Hin1I 312 GGCGTC G[GA]^CG[CT]C
777 GGCGCC
HphI 333 GGTGA GGTGA(8/7)
657 GGTGA
MaeII 749 ACGT A^CGT
869 ACGT
MspA1I 142 CAGCTG C[AC]G^C[GT]G
781 CCGCTG
NcoI 167 CCATGG C^CATGG
473 CCATGG
SapI 685 GAAGAGC GCTCTTC(1/4)
408 GCTCTTC
SfcI 105 CTATAG C^T[GA][CT]AG
493 CTGCAG
TaqI 185 TCGA T^CGA
446 TCGA
TthHB8I 185 TCGA T^CGA
446 TCGA
XmnI 120 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
438 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BsrBI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MaeIII MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI StyI TseI
Tsp45I