Restriction map for
pF1KB6332
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
Ksp632I KspI MamI MboI MfeI
MflI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 489 CCGC CCGC(-3/-1)
AcsI 788 GAATTT [GA]^AATT[CT]
ApoI 788 GAATTT [GA]^AATT[CT]
AspI 687 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 237 TGGCCA TGG^CCA
BfaI 76 CTAG C^TAG
BmyI 849 GGGCAC G[AGT]GC[ACT]^C
BsmI 28 GCATTC GAATGC(1/-1)
Bsp1286I 849 GGGCAC G[AGT]GC[ACT]^C
BstF5I 90 GGATG GGATG(2/0)
DraII 224 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 230 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 349 GACGGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 658 CCGTGG C^C[GA][CT]GG
EaeI 237 TGGCCA [CT]^GGCC[GA]
EcoO109I 224 AGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 90 GGATG GGATG(9/13)
HincII 814 GTTGAC GT[CT]^[GA]AC
HindII 814 GTTGAC GT[CT]^[GA]AC
HindIII 4 AAGCTT A^AGCTT
HphI 576 GGTGA GGTGA(8/7)
MaeI 76 CTAG C^TAG
MluNI 237 TGGCCA TGG^CCA
MscI 237 TGGCCA TGG^CCA
MseI 599 TTAA T^TAA
PflFI 687 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 224 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 257 CTGCAG CTGCA^G
SfcI 257 CTGCAG C^T[GA][CT]AG
SmlI 642 CTCAAG C^T[CT][GA]AG
SphI 750 GCATGC GCATG^C
Tru9I 599 TTAA T^TAA
Tth111I 687 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 94 GAAGAC GAAGAC(2/6)
676 GAAGAC
BglI 244 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
609 GCCTGGTGGGC
BpmI 464 CTCCAG CTGGAG(16/14)
520 CTGGAG
BpuAI 94 GAAGAC GAAGAC(2/6)
676 GAAGAC
BsmFI 226 GTCCC GGGAC(10/14)
723 GTCCC
Cac8I 296 GCACGC GC[ACGT]^[ACGT]GC
750 GCATGC
HaeIII 238 GGCC GG^CC
333 GGCC
HinfI 54 GAATC G^A[ACGT]TC
546 GAATC
MaeII 304 ACGT A^CGT
500 ACGT
MspA1I 260 CAGCTG C[AC]G^C[GT]G
833 CAGCTG
NlaIV 225 GGTCCC GG[ACGT]^[ACGT]CC
722 GGTCCC
NspI 98 ACATGC [GA]CATG^[CT]
750 GCATGC
PflMI 229 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCAGCATCTGG
PvuII 260 CAGCTG CAG^CTG
833 CAGCTG
TfiI 54 GAATC G^A[AT]TC
546 GAATC
Van91I 229 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCAGCATCTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwNI AvaII BbvI BsaJI
BseRI BsgI BsiYI BslI BsrI
BssKI BstNI Cfr13I DdeI EcoRII
EcoT22I Fnu4HI ItaI MaeIII MboII
MnlI MvaI MwoI NlaIII NsiI
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I TspRI