Restriction map for
pF1KB8500
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccII AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BcnI
BglI BlnI BsaAI BsaBI BsaI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspEI BsrDI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CpoI DraII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NciI NdeI
NheI NotI NruI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2344 GACGTC GACGT^C
AccI 925 GTCTAC GT^[AC][GT]AC
AflII 172 CTTAAG C^TTAAG
Alw44I 344 GTGCAC G^TGCAC
ApaLI 344 GTGCAC G^TGCAC
AseI 1831 ATTAAT AT^TAAT
AsnI 1831 ATTAAT AT^TAAT
BbsI 1362 GTCTTC GAAGAC(2/6)
BbvCI 1696 CCTCAGC CCTCAGC(-5/-2)
BcgI 2265 CGATGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 951 GTATCC GTATCC(6/5)
BfrI 172 CTTAAG C^TTAAG
BglII 1405 AGATCT A^GATCT
BlpI 2367 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2367 GCTGAGC GC^T[ACGT]AGC
BpuAI 1362 GTCTTC GAAGAC(2/6)
BsaWI 1672 ACCGGA [AT]^CCGG[AT]
BsgI 475 GTGCAG GTGCAG(16/14)
BsmAI 2550 GAGAC GTCTC(1/5)
BsmI 988 GAATGC GAATGC(1/-1)
BspDI 2263 ATCGAT AT^CGAT
BspHI 1899 TCATGA T^CATGA
BsrBI 1460 GAGCGG CCGCTC(-3/-3)
BssSI 913 CTCGTG CACGAG(-5/-1)
BstBI 238 TTCGAA TT^CGAA
CelII 2367 GCTGAGC GC^T[ACGT]AGC
ClaI 2263 ATCGAT AT^CGAT
DrdI 1390 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1559 CGGCCA [CT]^GGCC[GA]
EcoNI 291 CCTAACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 449 CCCGC CCCGC(4/6)
HaeII 1614 AGCGCC [GA]GCGC^[CT]
HgaI 1397 GCGTC GACGC(5/10)
HincII 1099 GTCAAC GT[CT]^[GA]AC
HindII 1099 GTCAAC GT[CT]^[GA]AC
NaeI 2393 GCCGGC GCC^GGC
NgoMIV 2393 GCCGGC G^CCGGC
NspV 238 TTCGAA TT^CGAA
PaeR7I 18 CTCGAG C^TCGAG
PshBI 1831 ATTAAT AT^TAAT
PvuII 730 CAGCTG CAG^CTG
RcaI 1899 TCATGA T^CATGA
SfuI 238 TTCGAA TT^CGAA
SpeI 2314 ACTAGT A^CTAGT
StuI 1265 AGGCCT AGG^CCT
XhoI 18 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1396 GGCGTC G[GA]^CG[CT]C
2344 GACGTC
AlwI 189 GGATC GGATC(4/5)
1200 GGATC
BanI 148 GGTGCC G^G[CT][GA]CC
1378 GGTGCC
BclI 411 TGATCA T^GATCA
2489 TGATCA
BsaHI 1396 GGCGTC G[GA]^CG[CT]C
2344 GACGTC
BsrFI 878 ACCGGC [GA]^CCGG[CT]
2393 GCCGGC
BstYI 189 GGATCT [GA]^GATC[CT]
1405 AGATCT
CfoI 504 GCGC GCG^C
1615 GCGC
Cfr10I 878 ACCGGC [GA]^CCGG[CT]
2393 GCCGGC
DraI 1062 TTTAAA TTT^AAA
1735 TTTAAA
DraIII 338 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2143 CACACCGTG
EarI 1274 CTCTTC CTCTTC(1/4)
757 GAAGAG
EcoRV 997 GATATC GAT^ATC
2080 GATATC
EcoT14I 1184 CCATGG C^C[AT][AT]GG
2532 CCATGG
EcoT22I 566 ATGCAT ATGCA^T
1159 ATGCAT
FbaI 411 TGATCA T^GATCA
2489 TGATCA
HhaI 504 GCGC GCG^C
1615 GCGC
Hin1I 1396 GGCGTC G[GA]^CG[CT]C
2344 GACGTC
HinP1I 504 GCGC G^CGC
1615 GCGC
HindIII 169 AAGCTT A^AGCTT
1045 AAGCTT
Ksp632I 1274 CTCTTC CTCTTC(1/4)
757 GAAGAG
MaeII 1241 ACGT A^CGT
2345 ACGT
MflI 189 GGATCT [GA]^GATC[CT]
1405 AGATCT
MslI 1494 CACTTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2493 CACCCACATG
MspA1I 730 CAGCTG C[AC]G^C[GT]G
1166 CAGCGG
NcoI 1184 CCATGG C^CATGG
2532 CCATGG
NsiI 566 ATGCAT ATGCA^T
1159 ATGCAT
PshAI 462 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1765 GACATTTGTC
SacI 15 GAGCTC GAGCT^C
333 GAGCTC
SmlI 18 CTCGAG C^T[CT][GA]AG
172 CTTAAG
SphI 1161 GCATGC GCATG^C
2100 GCATGC
StyI 1184 CCATGG C^C[AT][AT]GG
2532 CCATGG
XhoII 189 GGATCT [GA]^GATC[CT]
1405 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI