Restriction map for
pF1KB5035
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FbaI FseI FspI
HaeII HaeIII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvaI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 68 GCGG CCGC(-3/-1)
AcsI 285 GAATTT [GA]^AATT[CT]
AfaI 476 GTAC GT^AC
AlwI 376 GGATC GGATC(4/5)
AocI 340 CCTCAGG CC^T[ACGT]AGG
ApoI 285 GAATTT [GA]^AATT[CT]
AspHI 131 GAGCTC G[AT]GC[AT]^C
AvaI 280 CTCGAG C^[CT]CG[GA]G
BfaI 178 CTAG C^TAG
BglII 97 AGATCT A^GATCT
BpmI 543 CTCCAG CTGGAG(16/14)
BseRI 415 GAGGAG GAGGAG(10/8)
BsgI 189 CTGCAC GTGCAG(16/14)
BsiHKAI 131 GAGCTC G[AT]GC[AT]^C
BsmI 21 GCATTC GAATGC(1/-1)
BsoBI 280 CTCGAG C^[CT]CG[GA]G
BstF5I 429 GGATG GGATG(2/0)
BstYI 97 AGATCT [GA]^GATC[CT]
Bsu36I 340 CCTCAGG CC^T[ACGT]AGG
CfoI 141 GCGC GCG^C
DdeI 341 CTCAG C^T[ACGT]AG
DraI 309 TTTAAA TTT^AAA
DraII 336 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 340 CCTCAGG CC^T[ACGT]AGG
EcoO109I 336 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 453 CCATGG C^C[AT][AT]GG
FauI 68 GCGGG CCCGC(4/6)
FokI 429 GGATG GGATG(9/13)
HhaI 141 GCGC GCG^C
HinP1I 141 GCGC G^CGC
MaeI 178 CTAG C^TAG
MflI 97 AGATCT [GA]^GATC[CT]
NcoI 453 CCATGG C^CATGG
NlaIII 454 CATG CATG^
NlaIV 336 GGGTCC GG[ACGT]^[ACGT]CC
PaeR7I 280 CTCGAG C^TCGAG
PpuMI 336 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 328 CAGCTG CAG^CTG
RsaI 476 GTAC GT^AC
SacI 131 GAGCTC GAGCT^C
SfaNI 485 GCATC GCATC(5/9)
SmlI 280 CTCGAG C^T[CT][GA]AG
StyI 453 CCATGG C^C[AT][AT]GG
Tsp509I 286 AATT ^AATT
XhoI 280 CTCGAG C^TCGAG
XhoII 97 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 181 GGACC G^G[AT]CC
337 GGTCC
BanII 6 GAGCCC G[GA]GC[CT]^C
131 GAGCTC
BcnI 48 CCGGG CC^[GC]GG
194 CCCGG
BsaJI 34 CCACGG C^C[ACGT][ACGT]GG
453 CCATGG
BsiYI 32 CCCCACGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
517 CCGAGTATTGG
BslI 32 CCCCACGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
517 CCGAGTATTGG
BssKI 48 CCGGG ^CC[ACGT]GG
194 CCCGG
Cac8I 269 GCCAGC GC[ACGT]^[ACGT]GC
512 GCCTGC
Cfr13I 181 GGACC G^G[ACGT]CC
337 GGTCC
DsaI 34 CCACGG C^C[GA][CT]GG
453 CCATGG
HapII 48 CCGG C^CGG
195 CCGG
HpaII 48 CCGG C^CGG
195 CCGG
MboII 250 GAAGA GAAGA(8/7)
388 TCTTC
MseI 160 TTAA T^TAA
310 TTAA
MspA1I 66 CAGCGG C[AC]G^C[GT]G
328 CAGCTG
MspI 48 CCGG C^CGG
195 CCGG
NciI 48 CCGGG CC^[GC]GG
194 CCCGG
PstI 16 CTGCAG CTGCA^G
331 CTGCAG
Sau96I 181 GGACC G^G[ACGT]CC
337 GGTCC
ScrFI 48 CCGGG CC^[ACGT]GG
194 CCCGG
SfcI 16 CTGCAG C^T[GA][CT]AG
331 CTGCAG
TaqI 126 TCGA T^CGA
281 TCGA
Tru9I 160 TTAA T^TAA
310 TTAA
Tsp45I 491 GTGAC ^GT[GC]AC
530 GTGAC
TthHB8I 126 TCGA T^CGA
281 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BmyI Bsp1286I BsrI
DpnII Fnu4HI HinfI ItaI MaeIII
MboI MnlI MwoI NdeII Sau3AI
TfiI TseI TspRI