Restriction map for
pF1KB4540
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 151 GTAGAC GT^[AC][GT]AC
AflII 10 CTTAAG C^TTAAG
AflIII 883 ACATGT A^C[GA][CT]GT
AlwNI 282 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 690 ATTAAT AT^TAAT
AsnI 690 ATTAAT AT^TAAT
AspHI 55 GAGCTC G[AT]GC[AT]^C
AvaII 522 GGACC G^G[AT]CC
BanII 55 GAGCTC G[GA]GC[CT]^C
BbsI 1039 GAAGAC GAAGAC(2/6)
BclI 234 TGATCA T^GATCA
BcnI 551 CCCGG CC^[GC]GG
BfrI 10 CTTAAG C^TTAAG
BlpI 241 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 241 GCTCAGC GC^T[ACGT]AGC
BpuAI 1039 GAAGAC GAAGAC(2/6)
BsiEI 553 CGGCCG CG[GA][CT]^CG
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
BsmI 313 GAATGC GAATGC(1/-1)
BspLU11I 883 ACATGT A^CATGT
BsrFI 986 ACCGGC [GA]^CCGG[CT]
BstYI 142 GGATCT [GA]^GATC[CT]
CelII 241 GCTCAGC GC^T[ACGT]AGC
Cfr10I 986 ACCGGC [GA]^CCGG[CT]
Cfr13I 522 GGACC G^G[ACGT]CC
EagI 553 CGGCCG C^GGCCG
EclXI 553 CGGCCG C^GGCCG
Eco52I 553 CGGCCG C^GGCCG
EcoT22I 315 ATGCAT ATGCA^T
FbaI 234 TGATCA T^GATCA
HindIII 101 AAGCTT A^AGCTT
MflI 142 GGATCT [GA]^GATC[CT]
MslI 955 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 551 CCCGG CC^[GC]GG
NlaIV 582 GGAACC GG[ACGT]^[ACGT]CC
NsiI 315 ATGCAT ATGCA^T
PflMI 835 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 690 ATTAAT AT^TAAT
PstI 680 CTGCAG CTGCA^G
SacI 55 GAGCTC GAGCT^C
Sau96I 522 GGACC G^G[ACGT]CC
SfaNI 576 GATGC GCATC(5/9)
SfcI 680 CTGCAG C^T[GA][CT]AG
Tsp45I 676 GTGAC ^GT[GC]AC
Van91I 835 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 934 TCTAGA T^CTAGA
XcmI 381 CCAGATCAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 142 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 498 CCGC CCGC(-3/-1)
721 GCGG
BalI 393 TGGCCA TGG^CCA
440 TGGCCA
BmyI 55 GAGCTC G[AGT]GC[ACT]^C
429 GTGCCC
BpmI 508 CTCCAG CTGGAG(16/14)
609 CTCCAG
BsaI 19 GAGACC GGTCTC(1/5)
660 GGTCTC
BseRI 505 CTCCTC GAGGAG(10/8)
131 GAGGAG
Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C
429 GTGCCC
BspMI 713 ACCTGC ACCTGC(4/8)
641 GCAGGT
DdeI 71 CTCAG C^T[ACGT]AG
242 CTCAG
DsaI 596 CCATGG C^C[GA][CT]GG
654 CCATGG
EarI 16 GAAGAG CTCTTC(1/4)
787 GAAGAG
HapII 552 CCGG C^CGG
987 CCGG
HincII 645 GTTGAC GT[CT]^[GA]AC
1009 GTTGAC
HindII 645 GTTGAC GT[CT]^[GA]AC
1009 GTTGAC
HinfI 196 GAATC G^A[ACGT]TC
771 GATTC
HpaII 552 CCGG C^CGG
987 CCGG
HphI 675 GGTGA GGTGA(8/7)
973 TCACC
Ksp632I 16 GAAGAG CTCTTC(1/4)
787 GAAGAG
MaeIII 676 GTGAC ^GT[ACGT]AC
872 GTAAC
MluNI 393 TGGCCA TGG^CCA
440 TGGCCA
MscI 393 TGGCCA TGG^CCA
440 TGGCCA
MspA1I 565 CAGCTG C[AC]G^C[GT]G
612 CAGCTG
MspI 552 CCGG C^CGG
987 CCGG
NcoI 596 CCATGG C^CATGG
654 CCATGG
PvuII 565 CAGCTG CAG^CTG
612 CAGCTG
SspI 37 AATATT AAT^ATT
902 AATATT
TfiI 196 GAATC G^A[AT]TC
771 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BsaJI BsgI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII ItaI MaeI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NspI RsaI Sau3AI
ScrFI SmlI StyI Tru9I TseI
Tsp509I TspRI