Restriction map for
pF1KB3618
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MroI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I Tsp45I Tsp509I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 727 CGCG CG^CG
AcyI 730 GACGCC G[GA]^CG[CT]C
AfaI 681 GTAC GT^AC
AlwI 493 GATCC GGATC(4/5)
Aor51HI 719 AGCGCT AGC^GCT
ApaI 877 GGGCCC GGGCC^C
AspHI 61 GTGCTC G[AT]GC[AT]^C
AspI 446 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 564 TGGCCA TGG^CCA
BbvCI 375 CCTCAGC CCTCAGC(-5/-2)
BcgI 422 CGAACTGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 629 TGATCA T^GATCA
BglI 760 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 540 AGATCT A^GATCT
BsaBI 756 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 730 GACGCC G[GA]^CG[CT]C
BseRI 265 GAGGAG GAGGAG(10/8)
BsgI 392 CTGCAC GTGCAG(16/14)
BsiEI 16 CGACCG CG[GA][CT]^CG
BsiHKAI 61 GTGCTC G[AT]GC[AT]^C
BsrBI 19 CCGCTC CCGCTC(-3/-3)
BstAPI 538 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 727 CGCG CG^CG
BstYI 540 AGATCT [GA]^GATC[CT]
EaeI 564 TGGCCA [CT]^GGCC[GA]
Eco47III 719 AGCGCT AGC^GCT
FbaI 629 TGATCA T^GATCA
HaeII 719 AGCGCT [GA]GCGC^[CT]
Hin1I 730 GACGCC G[GA]^CG[CT]C
HinfI 30 GAATC G^A[ACGT]TC
MaeIII 161 GTAAC ^GT[ACGT]AC
MamI 756 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 540 AGATCT [GA]^GATC[CT]
MluNI 564 TGGCCA TGG^CCA
MscI 564 TGGCCA TGG^CCA
MvnI 727 CGCG CG^CG
PflFI 446 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 804 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 337 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
RsaI 681 GTAC GT^AC
ScaI 680 AGTACT AGT^ACT
SexAI 447 ACCAGGT A^CC[AT]GGT
SfcI 337 CTGCAG C^T[GA][CT]AG
SmlI 819 CTTGAG C^T[CT][GA]AG
TfiI 30 GAATC G^A[AT]TC
TspRI 515 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 446 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 804 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 540 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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BanII 435 GAGCCC G[GA]GC[CT]^C
877 GGGCCC
BbsI 109 GAAGAC GAAGAC(2/6)
649 GTCTTC
BpuAI 109 GAAGAC GAAGAC(2/6)
649 GTCTTC
BsmI 187 GCATTC GAATGC(1/-1)
287 GCATTC
CfoI 693 GCGC GCG^C
720 GCGC
EcoNI 777 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
798 CCTCCGCCAGG
HgaI 112 GACGC GACGC(5/10)
730 GACGC
HhaI 693 GCGC GCG^C
720 GCGC
HinP1I 693 GCGC G^CGC
720 GCGC
MspA1I 85 CCGCTG C[AC]G^C[GT]G
454 CAGCTG
PpuMI 67 AGGTCCC [GA]G^G[AT]CC[CT]
509 AGGTCCC
SfaNI 410 GCATC GCATC(5/9)
557 GCATC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI