Restriction map for
pF1KB5694
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AclI AcsI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FspI HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1033 GACGTC GACGT^C
Acc65I 497 GGTACC G^GTACC
AccIII 405 TCCGGA T^CCGGA
AlwI 1426 GATCC GGATC(4/5)
AocI 463 CCTGAGG CC^T[ACGT]AGG
Asp718I 497 GGTACC G^GTACC
AvaI 1117 CTCGAG C^[CT]CG[GA]G
BalI 1274 TGGCCA TGG^CCA
BbsI 643 GAAGAC GAAGAC(2/6)
BglI 1764 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 643 GAAGAC GAAGAC(2/6)
BsaI 1904 GAGACC GGTCTC(1/5)
BsaWI 405 TCCGGA [AT]^CCGG[AT]
BseAI 405 TCCGGA T^CCGGA
BsgI 1767 GTGCAG GTGCAG(16/14)
BsmBI 1805 CGTCTC CGTCTC(1/5)
BsoBI 1117 CTCGAG C^[CT]CG[GA]G
Bsp1407I 602 TGTACA T^GTACA
BspDI 1924 ATCGAT AT^CGAT
BspEI 405 TCCGGA T^CCGGA
BsrGI 602 TGTACA T^GTACA
BssSI 1459 CTCGTG CACGAG(-5/-1)
BstXI 1505 CCAACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1425 AGATCC [GA]^GATC[CT]
Bsu36I 463 CCTGAGG CC^T[ACGT]AGG
ClaI 1924 ATCGAT AT^CGAT
DsaI 773 CCGTGG C^C[GA][CT]GG
Eco81I 463 CCTGAGG CC^T[ACGT]AGG
EcoNI 1866 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 984 GATATC GAT^ATC
FseI 532 GGCCGGCC GGCCGG^CC
KpnI 497 GGTACC GGTAC^C
MflI 1425 AGATCC [GA]^GATC[CT]
MluNI 1274 TGGCCA TGG^CCA
MroI 405 TCCGGA T^CCGGA
MscI 1274 TGGCCA TGG^CCA
MslI 441 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 533 GCCGGC GCC^GGC
NgoMIV 533 GCCGGC G^CCGGC
PaeR7I 1117 CTCGAG C^TCGAG
PshAI 309 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1870 CTGCAG CTGCA^G
SacI 670 GAGCTC GAGCT^C
SalI 178 GTCGAC G^TCGAC
SbfI 1869 CCTGCAGG CCTGCA^GG
SfcI 1870 CTGCAG C^T[GA][CT]AG
Sse8387I 1869 CCTGCAGG CCTGCA^GG
SspBI 602 TGTACA T^GTACA
TfiI 1344 GAATC G^A[AT]TC
Tsp509I 1552 AATT ^AATT
XcmI 243 CCAGAGCGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1117 CTCGAG C^TCGAG
XhoII 1425 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 178 GTCGAC GT^[AC][GT]AC
394 GTCTAC
AcyI 1033 GACGTC G[GA]^CG[CT]C
1224 GGCGTC
AspHI 670 GAGCTC G[AT]GC[AT]^C
1443 GAGCAC
BanI 497 GGTACC G^G[CT][GA]CC
1367 GGCACC
BbvCI 168 CCTCAGC CCTCAGC(-5/-2)
1593 CCTCAGC
BlpI 82 GCTGAGC GC^T[ACGT]AGC
273 GCTCAGC
Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC
273 GCTCAGC
BsaHI 1033 GACGTC G[GA]^CG[CT]C
1224 GGCGTC
BsiHKAI 670 GAGCTC G[AT]GC[AT]^C
1443 GAGCAC
BsrFI 533 GCCGGC [GA]^CCGG[CT]
680 ACCGGC
CelII 82 GCTGAGC GC^T[ACGT]AGC
273 GCTCAGC
Cfr10I 533 GCCGGC [GA]^CCGG[CT]
680 ACCGGC
DrdI 582 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
901 GACAACGGGGTC
EaeI 531 TGGCCG [CT]^GGCC[GA]
1274 TGGCCA
EcoT14I 159 CCAAGG C^C[AT][AT]GG
1291 CCTTGG
FauI 1048 CCCGC CCCGC(4/6)
64 GCGGG
Hin1I 1033 GACGTC G[GA]^CG[CT]C
1224 GGCGTC
PpuMI 908 GGGTCCT [GA]G^G[AT]CC[CT]
1112 AGGACCT
PvuII 631 CAGCTG CAG^CTG
1261 CAGCTG
StyI 159 CCAAGG C^C[AT][AT]GG
1291 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I