Restriction map for
pF1KB5389
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1183 CGCG CG^CG
AclI 877 AACGTT AA^CGTT
AcsI 559 AAATTT [GA]^AATT[CT]
AflII 73 CTTAAG C^TTAAG
AflIII 1172 ACGTGT A^C[GA][CT]GT
Aor51HI 1130 AGCGCT AGC^GCT
ApaI 17 GGGCCC GGGCC^C
ApoI 559 AAATTT [GA]^AATT[CT]
Asp700I 283 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 864 GGTCC G^G[AT]CC
BamHI 246 GGATCC G^GATCC
BanI 646 GGCACC G^G[CT][GA]CC
BclI 194 TGATCA T^GATCA
BfrI 73 CTTAAG C^TTAAG
BglI 1292 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1274 ACCGGA [AT]^CCGG[AT]
BsmBI 950 GAGACG CGTCTC(1/5)
BspHI 737 TCATGA T^CATGA
BstEII 1161 GGTGACC G^GT[ACGT]ACC
BstPI 1161 GGTGACC G^GT[ACGT]ACC
BstUI 1183 CGCG CG^CG
BstYI 246 GGATCC [GA]^GATC[CT]
DraIII 1072 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1130 AGCGCT AGC^GCT
EcoO65I 1161 GGTGACC G^GT[ACGT]ACC
FbaI 194 TGATCA T^GATCA
HaeII 1130 AGCGCT [GA]GCGC^[CT]
HapII 1275 CCGG C^CGG
HpaII 1275 CCGG C^CGG
KspI 1182 CCGCGG CCGC^GG
MflI 246 GGATCC [GA]^GATC[CT]
MslI 1167 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1275 CCGG C^CGG
MvnI 1183 CGCG CG^CG
NcoI 326 CCATGG C^CATGG
NheI 579 GCTAGC G^CTAGC
Psp1406I 877 AACGTT AA^CGTT
PvuII 434 CAGCTG CAG^CTG
RcaI 737 TCATGA T^CATGA
SacII 1182 CCGCGG CCGC^GG
SapI 283 GAAGAGC GCTCTTC(1/4)
ScaI 926 AGTACT AGT^ACT
SexAI 1073 ACCAGGT A^CC[AT]GGT
StuI 965 AGGCCT AGG^CCT
TaqI 594 TCGA T^CGA
Tsp45I 1162 GTGAC ^GT[GC]AC
TthHB8I 594 TCGA T^CGA
XcmI 408 CCAATCTCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 246 GGATCC [GA]^GATC[CT]
XmnI 283 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 795 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
805 CAGATGCTG
BalI 2 TGGCCA TGG^CCA
449 TGGCCA
BbsI 138 GAAGAC GAAGAC(2/6)
832 GAAGAC
BfaI 580 CTAG C^TAG
1037 CTAG
BlpI 382 GCTGAGC GC^T[ACGT]AGC
456 GCTTAGC
Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC
456 GCTTAGC
BpuAI 138 GAAGAC GAAGAC(2/6)
832 GAAGAC
BsaI 904 GAGACC GGTCTC(1/5)
1261 GAGACC
BsgI 1270 CTGCAC GTGCAG(16/14)
939 GTGCAG
BsmFI 655 GGGAC GGGAC(10/14)
865 GTCCC
BspMI 471 ACCTGC ACCTGC(4/8)
1268 ACCTGC
BsrDI 365 CATTGC GCAATG(2/0)
996 CATTGC
CelII 382 GCTGAGC GC^T[ACGT]AGC
456 GCTTAGC
CfoI 1131 GCGC GCG^C
1284 GCGC
DraII 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
1307 GGGGCCT
EaeI 2 TGGCCA [CT]^GGCC[GA]
449 TGGCCA
EcoO109I 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
1307 GGGGCCT
EcoT14I 312 CCAAGG C^C[AT][AT]GG
326 CCATGG
FauI 1181 CCCGC CCCGC(4/6)
243 GCGGG
HhaI 1131 GCGC GCG^C
1284 GCGC
HinP1I 1131 GCGC G^CGC
1284 GCGC
MaeI 580 CTAG C^TAG
1037 CTAG
MaeIII 974 GTAAC ^GT[ACGT]AC
1162 GTGAC
MluNI 2 TGGCCA TGG^CCA
449 TGGCCA
MscI 2 TGGCCA TGG^CCA
449 TGGCCA
PflMI 855 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCACCAGGTGG
PstI 238 CTGCAG CTGCA^G
1118 CTGCAG
SacI 517 GAGCTC GAGCT^C
1054 GAGCTC
SfcI 238 CTGCAG C^T[GA][CT]AG
1118 CTGCAG
StyI 312 CCAAGG C^C[AT][AT]GG
326 CCATGG
TfiI 1248 GAATC G^A[AT]TC
1361 GAATC
Van91I 855 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCACCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
Tru9I TseI Tsp509I TspRI