Restriction map for
pF1KB5235
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Van91I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 712 GTAGAC GT^[AC][GT]AC
AhdI 724 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 781 CCTGAGG CC^T[ACGT]AGG
AspEI 724 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 805 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 974 CTCGAG C^[CT]CG[GA]G
BamHI 855 GGATCC G^GATCC
BanI 1128 GGTGCC G^G[CT][GA]CC
BciVI 829 GTATCC GTATCC(6/5)
BsaI 899 GAGACC GGTCTC(1/5)
BsiEI 636 CGATCG CG[GA][CT]^CG
BsmI 644 GCATTC GAATGC(1/-1)
BsoBI 974 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1322 TGTACA T^GTACA
BsrBI 1159 GAGCGG CCGCTC(-3/-3)
BsrGI 1322 TGTACA T^GTACA
BstAPI 4 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 813 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 781 CCTGAGG CC^T[ACGT]AGG
DrdI 616 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 495 TGGCCG [CT]^GGCC[GA]
Eam1105I 724 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 781 CCTGAGG CC^T[ACGT]AGG
EcoRI 508 GAATTC G^AATTC
HinfI 328 GACTC G^A[ACGT]TC
MaeII 233 ACGT A^CGT
MseI 440 TTAA T^TAA
NruI 216 TCGCGA TCG^CGA
NspI 1103 GCATGT [GA]CATG^[CT]
PaeR7I 974 CTCGAG C^TCGAG
PflFI 805 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 328 GACTC GAGTC(4/5)
PpuMI 135 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 190 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 636 CGATCG CGAT^CG
SacI 367 GAGCTC GAGCT^C
SapI 1045 GAAGAGC GCTCTTC(1/4)
ScaI 1358 AGTACT AGT^ACT
SspBI 1322 TGTACA T^GTACA
Tru9I 440 TTAA T^TAA
Tsp45I 1441 GTGAC ^GT[GC]AC
Tth111I 805 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 648 TCTAGA T^CTAGA
XcmI 813 CCAGCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 974 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 49 CGCG CG^CG
217 CGCG
AcsI 157 AAATTT [GA]^AATT[CT]
508 GAATTC
ApoI 157 AAATTT [GA]^AATT[CT]
508 GAATTC
AspHI 163 GTGCTC G[AT]GC[AT]^C
367 GAGCTC
BbsI 325 GAAGAC GAAGAC(2/6)
1474 GAAGAC
BcnI 200 CCCGG CC^[GC]GG
504 CCGGG
BlpI 1143 GCTCAGC GC^T[ACGT]AGC
1312 GCTGAGC
Bpu1102I 1143 GCTCAGC GC^T[ACGT]AGC
1312 GCTGAGC
BpuAI 325 GAAGAC GAAGAC(2/6)
1474 GAAGAC
BsgI 1244 CTGCAC GTGCAG(16/14)
263 GTGCAG
BsiHKAI 163 GTGCTC G[AT]GC[AT]^C
367 GAGCTC
BsrDI 531 CATTGC GCAATG(2/0)
418 GCAATG
BstUI 49 CGCG CG^CG
217 CGCG
BstYI 590 AGATCC [GA]^GATC[CT]
855 GGATCC
CelII 1143 GCTCAGC GC^T[ACGT]AGC
1312 GCTGAGC
EcoNI 621 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1479 CCTTGTGGAGG
EcoT14I 176 CCAAGG C^C[AT][AT]GG
884 CCTTGG
FauI 362 GCGGG CCCGC(4/6)
471 GCGGG
HapII 201 CCGG C^CGG
504 CCGG
HgaI 641 GACGC GACGC(5/10)
1082 GACGC
HindIII 334 AAGCTT A^AGCTT
1276 AAGCTT
HpaII 201 CCGG C^CGG
504 CCGG
MaeIII 1353 GTTAC ^GT[ACGT]AC
1441 GTGAC
MflI 590 AGATCC [GA]^GATC[CT]
855 GGATCC
MslI 993 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1149 CATTGGGGTG
MspA1I 130 CAGCTG C[AC]G^C[GT]G
844 CAGCTG
MspI 201 CCGG C^CGG
504 CCGG
MvnI 49 CGCG CG^CG
217 CGCG
NciI 200 CCCGG CC^[GC]GG
504 CCGGG
PvuII 130 CAGCTG CAG^CTG
844 CAGCTG
SfcI 192 CTATAG C^T[GA][CT]AG
1021 CTACAG
StuI 57 AGGCCT AGG^CCT
881 AGGCCT
StyI 176 CCAAGG C^C[AT][AT]GG
884 CCTTGG
Tsp509I 158 AATT ^AATT
509 AATT
XhoII 590 AGATCC [GA]^GATC[CT]
855 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HphI ItaI Ksp632I
MaeI MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TseI TspRI TthHB8I