Restriction map for
pF1KB5060
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# NO CUT ENZYMES ( 115 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AvaI AvrII BamHI
BbrPI BbvCI BcgI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1597 GACGTC GACGT^C
AccI 1441 GTCTAC GT^[AC][GT]AC
AccII 106 CGCG CG^CG
AcsI 1144 GAATTC [GA]^AATT[CT]
AhdI 304 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 188 GGGCCC GGGCC^C
ApoI 1144 GAATTC [GA]^AATT[CT]
AspEI 304 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 265 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 727 TGCGCA TGC^GCA
BbeI 88 GGCGCC GGCGC^C
BbsI 1125 GAAGAC GAAGAC(2/6)
BciVI 1203 GGATAC GTATCC(6/5)
BpuAI 1125 GAAGAC GAAGAC(2/6)
BsaBI 648 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 350 GAGAC GTCTC(1/5)
BsmBI 350 GAGACG CGTCTC(1/5)
BspMI 905 ACCTGC ACCTGC(4/8)
BstUI 106 CGCG CG^CG
Eam1105I 304 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1144 GAATTC G^AATTC
FauI 450 GCGGG CCCGC(4/6)
FspI 727 TGCGCA TGC^GCA
HaeII 88 GGCGCC [GA]GCGC^[CT]
KasI 88 GGCGCC G^GCGCC
MaeII 1598 ACGT A^CGT
MamI 648 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 106 CGCG CG^CG
NarI 88 GGCGCC GG^CGCC
NcoI 608 CCATGG C^CATGG
NspI 68 ACATGC [GA]CATG^[CT]
PflFI 265 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 712 CAGCTG CAG^CTG
SfiI 291 GGCCTCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 88 GGCGCC GGC^GCC
SspI 1322 AATATT AAT^ATT
TfiI 1629 GATTC G^A[AT]TC
Tsp509I 1145 AATT ^AATT
Tth111I 265 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 276 GTGCAC G^TGCAC
1271 GTGCAC
AlwI 1035 GATCC GGATC(4/5)
244 GGATC
AocI 1057 CCTGAGG CC^T[ACGT]AGG
1742 CCTCAGG
ApaLI 276 GTGCAC G^TGCAC
1271 GTGCAC
BanI 88 GGCGCC G^G[CT][GA]CC
942 GGTGCC
BglII 113 AGATCT A^GATCT
434 AGATCT
BsgI 366 CTGCAC GTGCAG(16/14)
481 GTGCAG
BsrDI 1545 CATTGC GCAATG(2/0)
572 GCAATG
BssSI 343 CACGAG CACGAG(-5/-1)
1105 CACGAG
Bsu36I 1057 CCTGAGG CC^T[ACGT]AGG
1742 CCTCAGG
CfoI 89 GCGC GCG^C
728 GCGC
DraIII 138 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1150 CACACTGTG
DsaI 608 CCATGG C^C[GA][CT]GG
971 CCGTGG
Eco81I 1057 CCTGAGG CC^T[ACGT]AGG
1742 CCTCAGG
HhaI 89 GCGC GCG^C
728 GCGC
HinP1I 89 GCGC G^CGC
728 GCGC
MslI 468 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CATGAGTGTG
MspA1I 448 CAGCGG C[AC]G^C[GT]G
712 CAGCTG
PleI 1128 GACTC GAGTC(4/5)
1386 GACTC
XcmI 209 CCACCCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
926 CCAGGAGCGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaII BalI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PpuMI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII