Restriction map for
pF1KB3065
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BamHI BbeI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 511 GTCTAC GT^[AC][GT]AC
AccIII 1223 TCCGGA T^CCGGA
ApaI 1068 GGGCCC GGGCC^C
AspHI 1353 GAGCAC G[AT]GC[AT]^C
AviII 1254 TGCGCA TGC^GCA
BalI 350 TGGCCA TGG^CCA
BbrPI 532 CACGTG CAC^GTG
BbsI 91 GAAGAC GAAGAC(2/6)
BclI 1055 TGATCA T^GATCA
BcnI 989 CCCGG CC^[GC]GG
BglI 697 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 394 CTGGAG CTGGAG(16/14)
BpuAI 91 GAAGAC GAAGAC(2/6)
BsaWI 1223 TCCGGA [AT]^CCGG[AT]
BseAI 1223 TCCGGA T^CCGGA
BsgI 1294 CTGCAC GTGCAG(16/14)
BsiHKAI 1353 GAGCAC G[AT]GC[AT]^C
BsmI 1138 GCATTC GAATGC(1/-1)
BspEI 1223 TCCGGA T^CCGGA
BspLU11I 881 ACATGT A^CATGT
BspMI 1208 ACCTGC ACCTGC(4/8)
BsrDI 354 CATTGC GCAATG(2/0)
BstAPI 1306 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1255 GCGC GCG^C
EarI 942 GAAGAG CTCTTC(1/4)
EcoNI 668 CCTATAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1055 TGATCA T^GATCA
FspI 1254 TGCGCA TGC^GCA
HgaI 416 GCGTC GACGC(5/10)
HhaI 1255 GCGC GCG^C
HinP1I 1255 GCGC G^CGC
Ksp632I 942 GAAGAG CTCTTC(1/4)
MluNI 350 TGGCCA TGG^CCA
MroI 1223 TCCGGA T^CCGGA
MscI 350 TGGCCA TGG^CCA
NciI 989 CCCGG CC^[GC]GG
PflMI 342 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1282 GACTC GAGTC(4/5)
PmaCI 532 CACGTG CAC^GTG
PmlI 532 CACGTG CAC^GTG
PpuMI 1205 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1029 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 346 CAGCTG CAG^CTG
SapI 942 GAAGAGC GCTCTTC(1/4)
SexAI 221 ACCAGGT A^CC[AT]GGT
StuI 448 AGGCCT AGG^CCT
TfiI 924 GATTC G^A[AT]TC
Van91I 342 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 139 AAATTC [GA]^AATT[CT]
238 GAATTT
AlwNI 343 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1258 CAGCCTCTG
ApoI 139 AAATTC [GA]^AATT[CT]
238 GAATTT
BanI 379 GGCACC G^G[CT][GA]CC
934 GGCACC
BanII 69 GGGCTC G[GA]GC[CT]^C
1068 GGGCCC
BfaI 13 CTAG C^TAG
208 CTAG
BsaAI 64 TACGTG [CT]AC^GT[GA]
532 CACGTG
BseRI 277 GAGGAG GAGGAG(10/8)
1197 GAGGAG
BsmAI 194 GAGAC GTCTC(1/5)
252 GTCTC
BstXI 692 CCAAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCAAGACCCTGG
DsaI 608 CCGTGG C^C[GA][CT]GG
698 CCGTGG
EaeI 350 TGGCCA [CT]^GGCC[GA]
991 CGGCCA
EcoT14I 692 CCAAGG C^C[AT][AT]GG
1370 CCAAGG
HapII 990 CCGG C^CGG
1224 CCGG
HinfI 924 GATTC G^A[ACGT]TC
1282 GACTC
HpaII 990 CCGG C^CGG
1224 CCGG
MaeI 13 CTAG C^TAG
208 CTAG
MseI 148 TTAA T^TAA
1124 TTAA
MslI 808 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1380 CATGTCTGTG
MspA1I 346 CAGCTG C[AC]G^C[GT]G
441 CCGCTG
MspI 990 CCGG C^CGG
1224 CCGG
NspI 881 ACATGT [GA]CATG^[CT]
1229 GCATGT
PstI 444 CTGCAG CTGCA^G
1143 CTGCAG
StyI 692 CCAAGG C^C[AT][AT]GG
1370 CCAAGG
TaqI 7 TCGA T^CGA
635 TCGA
Tru9I 148 TTAA T^TAA
1124 TTAA
TthHB8I 7 TCGA T^CGA
635 TCGA
XcmI 338 CCAGCCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
692 CCAAGGCCGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeIII HphI ItaI MaeII MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I Tsp509I TspRI