Restriction map for
pF1KB6826
##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MboI
MflI MluI MluNI MroI MscI
MslI MspI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 474 GTAC GT^AC
AlwNI 477 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 491 GGACC G^G[AT]CC
BbsI 357 GAAGAC GAAGAC(2/6)
BfaI 287 CTAG C^TAG
BpuAI 357 GAAGAC GAAGAC(2/6)
BsgI 421 CTGCAC GTGCAG(16/14)
BsiYI 213 CCAGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 213 CCAGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 410 TCATGA T^CATGA
BstEII 547 GGTTACC G^GT[ACGT]ACC
BstPI 547 GGTTACC G^GT[ACGT]ACC
Cfr13I 491 GGACC G^G[ACGT]CC
EcoO65I 547 GGTTACC G^GT[ACGT]ACC
EcoT14I 467 CCAAGG C^C[AT][AT]GG
HaeIII 465 GGCC GG^CC
HgaI 223 GACGC GACGC(5/10)
HphI 395 TCACC GGTGA(8/7)
MaeI 287 CTAG C^TAG
MfeI 130 CAATTG C^AATTG
MspA1I 199 CAGCTG C[AC]G^C[GT]G
MunI 130 CAATTG C^AATTG
PshAI 492 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 202 CTGCAG CTGCA^G
PvuII 199 CAGCTG CAG^CTG
RcaI 410 TCATGA T^CATGA
RsaI 474 GTAC GT^AC
Sau96I 491 GGACC G^G[ACGT]CC
SfaNI 246 GATGC GCATC(5/9)
SmlI 157 CTCAAG C^T[CT][GA]AG
StyI 467 CCAAGG C^C[AT][AT]GG
Tsp45I 479 GTCAC ^GT[GC]AC
XcmI 494 CCATAGTCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 184 CCTGAGG CC^T[ACGT]AGG
513 CCTAAGG
BpmI 250 CTCCAG CTGGAG(16/14)
379 CTGGAG
BsaJI 398 CCAGGG C^C[ACGT][ACGT]GG
467 CCAAGG
BseRI 16 CTCCTC GAGGAG(10/8)
187 GAGGAG
BsmAI 304 GAGAC GTCTC(1/5)
14 GTCTC
BsrI 292 ACTGG ACTGG(1/-1)
378 ACTGG
BstF5I 180 CATCC GGATG(2/0)
167 GGATG
Bsu36I 184 CCTGAGG CC^T[ACGT]AGG
513 CCTAAGG
DdeI 185 CTGAG C^T[ACGT]AG
514 CTAAG
Eco81I 184 CCTGAGG CC^T[ACGT]AGG
513 CCTAAGG
FokI 180 CATCC GGATG(9/13)
167 GGATG
MaeIII 479 GTCAC ^GT[ACGT]AC
548 GTTAC
MseI 194 TTAA T^TAA
533 TTAA
MwoI 120 GCTCCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
198 GCAGCTGCAGC
NlaIII 63 CATG CATG^
411 CATG
SfcI 50 CTACAG C^T[GA][CT]AG
202 CTGCAG
Tru9I 194 TTAA T^TAA
533 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BssKI BstNI EcoRII
Fnu4HI HinfI ItaI MboII MnlI
MvaI ScrFI TfiI TseI Tsp509I
TspRI