Restriction map for
pF1KB9104
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccIII AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 1195 GACGTC GACGT^C
AclI 426 AACGTT AA^CGTT
AcyI 1195 GACGTC G[GA]^CG[CT]C
AflIII 1252 ACACGT A^C[GA][CT]GT
AlwNI 823 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 683 CCCGAG C^[CT]CG[GA]G
BalI 110 TGGCCA TGG^CCA
BsaBI 1087 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1195 GACGTC G[GA]^CG[CT]C
BsaI 1387 GAGACC GGTCTC(1/5)
BsgI 178 GTGCAG GTGCAG(16/14)
BsoBI 683 CCCGAG C^[CT]CG[GA]G
BspHI 966 TCATGA T^CATGA
BsrBI 1144 GAGCGG CCGCTC(-3/-3)
BsrDI 581 GCAATG GCAATG(2/0)
BssHII 460 GCGCGC G^CGCGC
BstAPI 492 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI 108 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1208 GGCGCT [GA]GCGC^[CT]
HgaI 282 GACGC GACGC(5/10)
Hin1I 1195 GACGTC G[GA]^CG[CT]C
HincII 1309 GTCAAC GT[CT]^[GA]AC
HindII 1309 GTCAAC GT[CT]^[GA]AC
MamI 1087 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
NcoI 1571 CCATGG C^CATGG
Psp1406I 426 AACGTT AA^CGTT
RcaI 966 TCATGA T^CATGA
SmlI 604 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 571 GAGCTC G[AT]GC[AT]^C
1558 GAGCTC
AviII 380 TGCGCA TGC^GCA
578 TGCGCA
BamHI 25 GGATCC G^GATCC
1086 GGATCC
BglI 40 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
232 GCCGAGCTGGC
BlpI 211 GCTGAGC GC^T[ACGT]AGC
487 GCTCAGC
Bpu1102I 211 GCTGAGC GC^T[ACGT]AGC
487 GCTCAGC
BsaAI 1253 CACGTA [CT]AC^GT[GA]
1357 TACGTG
BsiEI 1129 CGGCCG CG[GA][CT]^CG
1340 CGGCCG
BsiHKAI 571 GAGCTC G[AT]GC[AT]^C
1558 GAGCTC
BsmI 948 GAATGC GAATGC(1/-1)
464 GCATTC
CelII 211 GCTGAGC GC^T[ACGT]AGC
487 GCTCAGC
EagI 1129 CGGCCG C^GGCCG
1340 CGGCCG
EclXI 1129 CGGCCG C^GGCCG
1340 CGGCCG
Eco52I 1129 CGGCCG C^GGCCG
1340 CGGCCG
EcoT14I 113 CCAAGG C^C[AT][AT]GG
1571 CCATGG
FspI 380 TGCGCA TGC^GCA
578 TGCGCA
MboII 1065 GAAGA GAAGA(8/7)
68 TCTTC
PflMI 767 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCAGCTGCTGG
PstI 50 CTGCAG CTGCA^G
565 CTGCAG
SacI 571 GAGCTC GAGCT^C
1558 GAGCTC
SfcI 50 CTGCAG C^T[GA][CT]AG
565 CTGCAG
SphI 781 GCATGC GCATG^C
998 GCATGC
StuI 495 AGGCCT AGG^CCT
714 AGGCCT
StyI 113 CCAAGG C^C[AT][AT]GG
1571 CCATGG
Van91I 767 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCAGCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII